Zinc-polypeptides complexe and its preparation method and application

A technology of polypeptide complexes and complexes, applied in the field of chemistry, can solve the problems of high difficulty, limitation and high cost of chemical synthesis, and achieve the effects of improving immunity, enhancing immunity and low price.

Inactive Publication Date: 2008-10-15
GUANGZHOU UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Because the chemical synthesis of pure peptides is difficult and costly, and the biological activity of chemically synthesized peptides is often lower than that of peptid

Method used

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  • Zinc-polypeptides complexe and its preparation method and application
  • Zinc-polypeptides complexe and its preparation method and application
  • Zinc-polypeptides complexe and its preparation method and application

Examples

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example 1

[0067] Pretreatment of animal raw materials: Weigh the leftovers of tilapia processing, and use a tissue grinder or chopping machine to quickly chop them into fine pieces to avoid the raw materials from forming a gel.

[0068] Protein hydrolysis: take a certain amount of raw materials, according to the material: water = 1:3, dissolve in water, stir, adjust the pH to 9.0, add alkaline protease 700U / g raw materials, and perform enzymatic hydrolysis reaction in a 50°C constant temperature water bath shaking shaker for 2 hours , shaking once every 10 minutes. Then the hydrolyzed solution was heated to 100°C, kept for 10 min, cooled to room temperature, filtered to obtain the clear solution for later use. The alkaline protease used in the hydrolysis was Alcalase 2.4L FG, provided by Novozymes Biotechnology Co., Ltd.

[0069] Complex preparation: Take 100ml of clear liquid, adjust the pH to 4.0, add 10ml of zinc chloride solution with a concentration of 0.1g / ml, preheat the electri...

example 2

[0082] Pretreatment of animal raw materials: Weigh the leftovers of tilapia processing, and use a tissue grinder or chopping machine to quickly chop them into fine pieces to avoid the raw materials from forming a gel.

[0083]Protein hydrolysis: take a certain amount of raw materials, according to the material: water = 1:5, dissolve in water, stir, adjust the pH to 7.5, add Bacillus subtilis neutral protease 1000U / g raw materials, and perform enzymolysis in a constant temperature water bath shaking shaker at 55°C React for 3 hours, shaking every 10 minutes. Then the hydrolyzate was heated to 100°C, kept for 10 min, cooled to room temperature, and centrifuged to obtain the supernatant for later use. The Bacillus subtilis neutral protease used in the hydrolysis was provided by Nanning Pangbo Bioengineering Co., Ltd., Guangxi.

[0084] Complex preparation: Take 100ml of clear liquid, adjust the pH to 4.0, add 10ml of zinc chloride solution with a concentration of 0.1g / ml, prehea...

example 3

[0086] Pretreatment of animal raw materials: Weigh the leftovers of tilapia processing, and use a tissue grinder or chopping machine to quickly chop them into fine pieces to avoid the raw materials from forming a gel.

[0087] Protein hydrolysis: take a certain amount of raw materials, according to the material: water = 1: 4, dissolve in water, stir, adjust the pH to 9.0, add alkaline protease 8000U / g raw materials, and carry out enzymatic hydrolysis reaction in a 55°C constant temperature water bath shaking shaker 2.5 h, shaking once every 10min. Then the hydrolyzed solution was heated to 100°C, kept for 10 min, cooled to room temperature, filtered to obtain the clear solution for later use. The alkaline protease used in the hydrolysis was Alcalase 2.4L FG, provided by Novozymes Biotechnology Co., Ltd.

[0088] Complex preparation: Take 100ml of clear liquid, adjust the pH to 5.0, add 10ml of zinc oxide solution with a concentration of 0.1g / ml, preheat the electric furnace f...

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Abstract

The invention provides a zinc-polypeptide composition, which is prepared from a zinc mineral and an animal material in a weight ratio of (1:1) to (1:5) by hydrolyzing the animal material by protease to obtain polypeptide, and complex-reacting with the zinc mineral. The composition not only contains zinc elements absorbable by human and animal bodies but contains polypeptide component, thus supplementing zinc and enhancing immunity. The composition can be used as a food, a nutritional product, a healthy product or an animal feed additive for supplementing zinc and improving immunity. The materials are selected from natural animal materials, which has low cost, such as low-value agricultural and fishery products or scraps or leftovers of agricultural and fishery products.

Description

technical field [0001] The invention relates to the field of chemistry, in particular to metal polypeptide complexes. Background technique [0002] Zinc (Zn) is an essential trace element for humans and animals, known as the element of life. At present, organic zinc is mainly amino acid chelate, such as amino acid chelate of trace elements such as zinc, selenium or calcium. Amino acid chelates that consume trace elements can not only supplement the trace elements required by the human body or animals, but also supplement the amino acids required by the human body or animals at the same time. The State Intellectual Property Bureau published an invention patent application "Preparation method and application of amino acid zinc" (application number 94114854.8) on April 2, 1997. This application discloses a preparation method of triglycine zinc chelate, which is Amino acid and zinc oxide are mixed at a molar ratio of 2:1, concentrated in an aqueous solution for a period of tim...

Claims

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Application Information

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IPC IPC(8): A23L1/30A23L1/305A23L1/304A23L1/327A23L1/33A23K1/16A23K1/175A23K1/10A23K10/14A23K10/26A23K20/147A23K20/20A23L33/10A23L33/165A23L33/18
Inventor 曾庆祝许庆陵
Owner GUANGZHOU UNIVERSITY
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