Hematid immunity function test method

A detection method and technology of immune function, applied in the field of medical detection, can solve the problems affecting the reliability of detection results, high preparation cost, waste of raw materials, etc., and achieve the effects of true and reliable detection results, accurate and reliable detection results, and easy popularization and promotion.

Inactive Publication Date: 2008-10-29
常国良
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AI Technical Summary

Problems solved by technology

However, in the detection test and clinical application, it is found that Guo Feng's improved method still has the following disadvantages: 1. The phenomenon of complement inactivation is serious, and the reagent storage period is short, which affects the reliability of the detection results; 2. The preparation method of the detection reagent is cumbersome and the raw materials The waste is large, and the preparation cost is high; 3. The operation of the detection test is also relatively cumbersome

Method used

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Examples

Experimental program
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Embodiment Construction

[0020] 1. Preparation of cell suspension

[0021] Take 1-2mL of fasting venous blood from the subjects, anticoagulate with heparin, equilibrate with normal saline, centrifuge at 2500r / min for 5min, extract plasma into a test tube, and mark it for later use.

[0022] Inject about 4mL of normal saline into another test tube, extract 10uL of whole blood cells from the middle layer of the separated whole blood cells, add it into the normal saline test tube, pipette evenly, count red blood cells under a microscope, and prepare a red blood cell count of 1.25×10 7 / mL of whole blood cell suspension and marked for later use.

[0023] 2. Preparation of yeast solution

[0024] 1.0×10 8 The / mL yeast solution was provided by the Blood Immunology Laboratory of Changhai Hospital, Second Military Medical University, Shanghai.

[0025] Cut the frozen yeast solution, thaw it at room temperature, blow it until it is uniform, and then use it.

[0026] 3. Detection test

[0027] 1) Take 2 t...

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PUM

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Abstract

The invention discloses a detection method for red blood cell immune function, comprising the following steps: (1) heparin anticoagulant blood is taken to carriy out centrifugalization, blood plasma is extracted for spare, and red blood cells are prepared into cell suspension of 1.25 multiplied by 10<7> / mL; (2) yeast is prepared into yeast solution of 1.0 multiplied by 10<8> / mL ; (3) the cell suspension and the yeast solution with the equal volume are mixed to be even, the blood plasma is added / not added, then water bath with the temperature of 37 DEG C is carried out for 30minutes, glutaric dialdehyde is fixed, smear, drying and dying are carried out, 100 red blood cells are counted, red blood cells stuck with two or more than two yeasts are one positive rosette, and the rosette rates of red blood cell C3bR and IC are calculated; the invention uses the self-blood plasma to carry out yeast sensitization with quick taking and using, no complement is inactive, no allogenic blood ingredients interfere, the preparation of the sensitized yeast reagent is not needed, complete blood cells are used for preparing the cell suspension after the blood plasma is extracted, thus truly reflecting the environment condition in the body, and leading the test cost to be reduced obviously and the detected result to be accurate and reliable.

Description

technical field [0001] The invention belongs to the technical field of medical detection, and relates to a detection method of erythrocyte immune function, in particular to a detection method of erythrocyte C3bR rosette test and erythrocyte IC rosette test. Background technique [0002] Since the red blood cell was discovered in 1658, it has been considered as a transporter of oxygen and carbon dioxide. With the advancement of science and technology for more than 300 years, people have gradually realized more functions of red blood cells, among which the immune function of red blood cells is a major aspect. [0003] In 1981, American reproductive immunologist Siegel discovered a variety of immune functions of red blood cells on the basis of previous research results. He first proposed that red blood cells are immune cells and a subsystem of the immune system, and proposed "red blood cell immunity". The new concept of "system" has updated people's understanding of the functi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/556
Inventor 常国良
Owner 常国良
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