Method for preparing endotoxin adsorbing agent

An adsorbent and endotoxin technology, applied in chemical instruments and methods, suction devices, and other chemical processes, can solve problems such as risk of use, easy generation of particles, and large damage to the strength of agarose gel

Active Publication Date: 2010-09-29
JAFRON BIOMEDICAL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Such conditions greatly damage the strength of the agarose gel as the matrix, making the endotoxin adsorbent prone to produce particles during hemoperfusion, which brings risks to the use

Method used

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  • Method for preparing endotoxin adsorbing agent

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] A. Trichlorotriazine activation of spherical porous cellulose

[0044] First vacuum filter the spherical porous cellulose ball to remove the surface moisture.

[0045] Add 50 grams of spherical porous cellulose balls to the three-necked flask, add 100 milliliters of dichloroethane, adjust the temperature of the system to 4 ± 1 ° C, stir well, slowly add 18.2 grams of trichlorotriazine and 15.8 milliliters of N, N- After the dropwise addition of the mixed solution of isopropylformamide and 100 milliliters of dichloroethane was completed, the stirring reaction was continued for 4 hours.

[0046] After the reaction is completed, the reaction residual liquid is filtered out, and the activated spherical porous cellulose spheres are washed several times with cold anhydrous ether, and then washed with a large amount of purified water, and filtered to obtain the activated spherical porous cellulose, which is ready for use.

[0047] B. Coupling of activated spherical porous cel...

Embodiment 2

[0055] A. Trichlorotriazine activation of spherical porous cellulose

[0056] First vacuum filter the spherical porous cellulose ball to remove the surface moisture.

[0057] Add 50 grams of spherical porous cellulose balls to the three-necked flask, add 100 milliliters of dichloroethane, adjust the temperature of the system to 1 ± 1 ° C, stir well, slowly add 22.8 grams of trichlorotriazine and 14 milliliters of N, N- The mixed solution of diisopropylformamide and 100 milliliters of dichloroethane, after the dropwise addition was completed, continued to stir and react for 6 hours. After the reaction is completed, the reaction residue is filtered out, the activated spherical porous cellulose is washed several times with cold anhydrous ether, and then washed with a large amount of purified water, and filtered to obtain the activated spherical porous cellulose, which is set aside.

[0058] B. Coupling of activated spherical porous cellulose with triethyl phosphite

[0059] Add...

Embodiment 3

[0066] A. Trichlorotriazine activation of spherical porous cellulose

[0067] First vacuum filter the spherical porous cellulose ball to remove the surface moisture.

[0068] Add 50 grams of spherical porous cellulose balls to the three-necked flask, add 100 milliliters of dichloroethane, adjust the temperature of the system to 3±1°C, and after stirring evenly, slowly add 11.4 grams of trichlorotriazine and 12 milliliters of N,N- The mixed solution of diisopropylformamide and 100 milliliters of dichloroethane, after the dropwise addition was completed, continued to stir and react for 2 hours. After the reaction is completed, the reaction residue is filtered out, the activated spherical porous cellulose is washed several times with cold anhydrous ether, and then washed with a large amount of purified water, and filtered to obtain the activated spherical porous cellulose, which is set aside.

[0069] B. Coupling of activated spherical porous cellulose with triethyl phosphite

...

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Abstract

The invention relates to a preparation method of an endotoxin adsorbent. The spherical porous cellulose is taken as a carrier; the spherical porous cellulose is activated by triazine trichloride, coupled with triethyl phosphate, then immobilized polymyxin B, thus the endotoxin adsorbent is obtained. The endotoxin adsorbent obtained by the method has stable performance and good adsorption property.

Description

technical field [0001] The invention relates to an endotoxin adsorbent and a preparation method thereof, in particular to an endotoxin adsorbent for hemoperfusion and a preparation method thereof. Background technique [0002] Endotoxin is a kind of lipopolysaccharide produced by cleavage of the cell wall when Gram-negative bacteria are released when they grow or die. A very small amount (nanogram level) of endotoxin entering the human body will cause high fever, dilation of blood vessels, and even fainting or death . Endotoxemia is one of the most common clinical fatal diseases, and the mortality rate can reach 40%-90%. [0003] There is no clinically effective method for treating endotoxemia, and one of the existing methods is hemoperfusion through an adsorbent bound to polymyxin (PMB). Among them, polymyxin is a cyclic decapeptide, which is the N-terminus of cyclic structure 7-peptide and 3-peptide, containing lipophobic and lipophilic groups and a polypeptide tail. Po...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): B01J20/30A61M1/34A61M1/00
Inventor 董凡
Owner JAFRON BIOMEDICAL
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