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Fish collagen peptides ion exchange chromatography fishy smell elimination process

A technology of ion exchange chromatography and fish collagen peptide, which is applied in the field of removing fish collagen peptide fishy smell, and can solve problems such as collagen peptide fishy smell

Inactive Publication Date: 2013-02-27
青岛博恩生物科技有限公司
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Problems solved by technology

[0003] In order to solve the problem of fishy smell of collagen peptides, the present invention invents a technique for separating collagen peptides from fishy smell substances by ion exchange chromatography

Method used

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Embodiment Construction

[0006] Pretreatment and transformation of carboxymethyl cellulose ion exchanger: fully swell the dry powder carboxymethyl cellulose ion exchanger in deionized water, and use vacuum to suck the ion exchanger together with deionized water into the ion exchange column. The diameter-to-height ratio of the exchange column is 1:10, and the column volume is preferably 70% of the volume of the exchange column, and then suspended with water to remove impurities and fine particles. Soak with acid and alkali (acid first, then alkali) respectively, and finally wash with water until neutral. This is to further remove impurities and bring the required counter ions to the ion exchanger. After washing, let the liquid level be higher than the exchanger. Surface 10-15cm ready for use. The commonly used acid is HCl, the base is NaOH or a certain amount of NaCl is added, so that the ion exchanger after treatment is Na type, the acid-base concentration used is generally 1.0mol / L, and the soaking t...

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Abstract

The invention relates to a fish collagen peptide ion exchange chromatography and deodorization technique, which is characterized by comprising pretreatment, transformation, on-column and elution, wherein a carboxymethyl cellulose ion exchanger undergoes acid and alkali treatment and is then transformed into Na type; the pH of a collagen peptide solution with a concentration of between 8 and 10 percent is adjusted to between 3.5 and 4.0, and the collagen peptide solution passes through a carboxymethyl cellulose exchange column, and then collagen peptide and odorous materials are separated; the carboxymethyl cellulose exchange column is washed by pure water, and eluent is collected by means of sublevel so as to achieve the aim of separating the odorous materials and the collagen peptide. The technique solves the problem that the collagen peptide contains odor, and is suitable for being widely used in the production industry taking the collagen peptide as a raw material.

Description

technical field [0001] The invention mainly relates to the field of deep processing of seafood, in particular to a technology for removing the fishy smell of fish collagen peptides. Background technique [0002] At present, the collagen peptides produced from fish skin and fish scales both at home and abroad have the disadvantage of not being able to effectively remove the fishy smell, which limits the application field of fish collagen peptides. Contents of the invention [0003] In order to solve the problem of fishy smell of collagen peptide, the present invention invents a technology for separating collagen peptide and fishy smell substance by ion exchange chromatography. [0004] The technical solution of the present invention is a deodorization process of fish collagen peptide ion exchange chromatography, its special features include: pretreatment and transformation, column loading, elution; pretreatment and transformation: treatment of carboxymethyl fiber with acid ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K1/18
Inventor 王茜成徐强孙国强
Owner 青岛博恩生物科技有限公司
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