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Deodorization process for fish collagen peptide by utilizing ion exchange chromatography

A technology of ion exchange chromatography and fish collagen peptide, which is applied in the field of removing fish collagen peptide fishy smell, and can solve problems such as collagen peptide fishy smell

Inactive Publication Date: 2018-12-04
吴相云
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Problems solved by technology

[0003] In order to solve the problem of fishy smell of collagen peptides, the present invention invents a technique for separating collagen peptides from fishy smell substances by ion exchange chromatography

Method used

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Embodiment Construction

[0006] Pretreatment and transformation of carboxymethyl cellulose ion exchanger: fully swell the dry powder carboxymethyl cellulose ion exchanger in deionized water, and use vacuum to suck the ion exchanger together with deionized water into the ion exchange column. The diameter-to-height ratio of the exchange column is 1:10, and the column volume is preferably 70% of the volume of the exchange column, and then suspended with water to remove impurities and fine particles. Soak with acid and alkali (acid first, then alkali) respectively, and finally wash with water until neutral. This is to further remove impurities and bring the ion exchanger with the required counter ions. After washing, let the liquid level be higher than the exchanger. Surface 10-15cm ready for use. The commonly used acid is HCl, the base is NaOH or a certain amount of NaCl is added, so that the ion exchanger after treatment is Na type, the acid-base concentration used is generally 1.0 mol / L, and the soak...

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Abstract

Special features of a deodorization process for fish collagen peptide by utilizing ion exchange chromatography include pretreatment and transformation, injection of chromatographic column and elution.A carboxymethyl cellulose ion exchanger is treated by utilizing acid and alkali, and is transformed into a Na type; the pH of a collagen peptide solution at a concentration of 8-10% is adjusted to 3.5-4.0, the collagen peptide solution passes through a carboxymethyl cellulose exchange column, and the collagen peptide and fishy substances are separated; and the carboxymethyl cellulose exchange column is rinsed with pure water, and an eluent is collected in a segmentation manner until the fishy substances and the collagen peptide are separated. The deodorization process solves the problem thatthe collagen peptide contains fishiness, and is suitable for wide application in a production industry using the collagen peptide as a raw material.

Description

technical field [0001] The invention mainly relates to the field of deep processing of seafood, in particular to a technology for removing the fishy smell of fish collagen peptides. Background technique [0002] At present, the collagen peptides produced from fish skin and fish scales both at home and abroad have the disadvantage of not being able to effectively remove the fishy smell, which limits the application field of fish collagen peptides. Contents of the invention [0003] In order to solve the problem of fishy smell of collagen peptide, the present invention invents a technology for separating collagen peptide and fishy smell substance by ion exchange chromatography. [0004] The technical solution of the present invention is a deodorization process of fish collagen peptide ion exchange chromatography, its special features include: pretreatment and transformation, column loading, elution; pretreatment and transformation: treatment of carboxymethyl fiber with...

Claims

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Application Information

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IPC IPC(8): C07K14/78C07K1/18
CPCC07K14/78
Inventor 吴相云
Owner 吴相云
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