Method of assessing colorectal cancer by measuring hemoglobin and M2-PK in a stool sample

A colorectal cancer, hemoglobin technology, applied in the field of helping to evaluate colorectal cancer, can solve the problem of limited sensitivity of colon cancer

Inactive Publication Date: 2009-01-07
F HOFFMANN LA ROCHE & CO AG
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the test appears to have only limited sensitivity for detecting colon cancer on the right side (Davies, R.J., et al., Lancet 359 (2002) 1917-1919)

Method used

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  • Method of assessing colorectal cancer by measuring hemoglobin and M2-PK in a stool sample
  • Method of assessing colorectal cancer by measuring hemoglobin and M2-PK in a stool sample

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0101] research group

[0102] Stool samples from 94 well-characterized CRC patients using the UICC classification given in Table 1 were used.

[0103] Table 1: CRC samples and corresponding UICC classifications

[0104] Phases according to UICC

Number of samples

UICC 0

2

UICC I

21

UICC II

27

UICC III

36

UICC I-III (non-IV, categories I to III are not individually phased)

8

Total number of CRCs

94

[0105] The CRC samples of Table 1 have been evaluated compared to control samples obtained from individuals who underwent colonoscopy and did not have adenomas, polyps, or colorectal cancer. Table 2 gives an overview of the controls used:

[0106] Types of control patients

Embodiment 2

[0108] Extraction of stool samples for determination of hemoglobin and M2-PK

[0109] For each patient studied, two stool aliquots were collected at different locations on a single bolus prior to colonoscopy or surgery. A total of about 1 gram per stool sample was collected using specific collection equipment from Sarstedt (Id. no. 80.623.022). Fecal samples thus collected were frozen as soon as possible and stored at -70°C until extraction.

[0110] For the determination of hemoglobin, 100 mg of stool samples were placed in 2 mL Eppendorf cups for each extraction experiment. The 100 mg stool samples were extracted by using 1 ml fresh extraction buffer.

[0111] Use the following extraction buffers:

[0112] 9,49g Na 2 HPO 4 ×2H 2 o

[0113] 1,84g KH 2 PO 4

[0114] 1g NaN 3

[0115] 0,4g Na 2 EDTA×2H 2 o

[0116] 10ml chicken albumin

[0117] 50ml Nonidet P40 10% w / v

[0118] 1 tablet Complete mini (Roche Diagnostics, Id.No.1836145) was added to 1 L with doub...

Embodiment 3

[0122] Immunoassay for Determination of Hemoglobin and M2-PK from Extracts of Fecal Samples

[0123] 3.1 Hemoglobin

[0124] Hemoglobin determination was performed using the "Haem Immun" assay (Labor Limbach, Heidelberg) according to the manufacturer's instructions. 10 [mu]l of Hb extract was used as sample in the immunoassay.

[0125] 3.2M2-PK

[0126] The determination of M2-PK was used according to the instructions given by the manufacturer " Tumor M2-PK" assay (Schebo Biotech AG, Giessen). 50 μl of M2-PK extract was used as sample in this immunoassay.

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Abstract

The present invention relates to a method aiding in the assessment of colorectal cancer. The method especially is used in assessing the absence or presence of colorectal cancer in vitro. The method is for example practiced by analyzing biochemical markers, comprising measuring in a stool sample the concentration of hemoglobin and M2-PK and correlating the concentrations determined to the absence or presence of colorectal cancer. To further improve the assessment of colorectal cancer in a method of this invention the level of one or more additional marker may be determined together with hemoglobin and M2-PK in a stool sample and be correlated to the absence or presence of colorectal cancer. The invention also relates to the use of a marker panel comprising hemoglobin and M2-PK in the early diagnosis of colorectal cancer and it teaches a kit for performing the method of the invention.

Description

[0001] The present invention relates to methods to aid in the assessment of colorectal cancer. The method is especially useful for assessing the absence or presence of colorectal cancer in vitro. For example, the method is practiced by analyzing biochemical markers, including measuring the concentrations of hemoglobin and M2-PK in a stool sample, and correlating the determined concentrations with the absence or presence of colorectal cancer. To further improve the assessment of colorectal cancer in the methods of the invention, the levels of one or more additional markers can be determined together with hemoglobin and M2-PK in the stool sample and correlate with the absence or presence of colorectal cancer couplet. The invention also relates to the use of a marker panel comprising hemoglobin and M2-PK in the early diagnosis of colorectal cancer, which teaches kits for performing the method of the invention. Background of the invention [0002] Stool or stool samples are comm...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/574G01N33/68
CPCG01N2333/91215G01N33/721G01N33/57419
Inventor J·卡尔
Owner F HOFFMANN LA ROCHE & CO AG
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