Method for simultaneously separating soy protein and oil fat with inverse micelle abstraction technique
A simultaneous separation and soybean protein technology, applied in the field of separation of soybean protein and oil, can solve the problems of unstable product quality, large acid and alkali consumption, high cost, etc., to reduce production costs and fixed asset investment, and simplify the production process , to maintain the effect of biological activity
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[0036] Example 1
[0037] Add 80g of pretreated full-fat soybean meal to 4L 80g / L AOT reverse micelle solution, control the conditions, the buffer in the reverse micelle solution is NaNO 3 -Phosphate buffer, control condition W 0 9, NaNO 3 The concentration is 0.05mol / L, the pH is adjusted to 8.0, the extraction temperature is 45°C, and the mechanical stirring is performed at a speed of 180r / min for 2h. The protein pre-extraction rate is 60.0%.
[0038] Add an equal volume of 1.0mol / L NaCl-phosphate buffer, adjust the pH value to 7.7, shake for 1.5h at a speed of 300r / min and centrifuge for 0.5h, statically layer to obtain the upper layer of mixed oil, the lower layer of protein aqueous solution. The protein post-extraction rate was 87.1%. The oil extraction rate is 90.0%.
[0039] Overall, the protein extraction rate was 52.3%, and the oil extraction rate was 90.0%. The foamability, emulsification and emulsification stability of soy protein are better than those produced by alkal...
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[0040] Example 2
[0041] Add 0.3g of pretreated full-fat soybean meal to 20mL of reverse micelle solution with an AOT concentration of 120g / L. The buffer in the reverse micelle solution is KCl-phosphate buffer, and the control condition is W 0 18. The KCl concentration is 0.05mol / L, the pH is adjusted to 7.5, the extraction temperature is 40°C, and it is shaken at a speed of 180r / min for 1h. The protein pre-extraction rate is 64.6%.
[0042] Add an equal volume of 1.0mol / L KCl-phosphate buffer solution, adjust the pH value to 7.0, oscillate in a shaker at a speed of 250r / min or more for 2h, then centrifuge for 15min, static layering to obtain the upper layer of mixed oil, the lower layer of protein aqueous solution. The protein post-extraction rate is 89.0%. The oil extraction rate is 86.0%. Overall, the protein extraction rate was 57.5%, and the oil extraction rate was 86.0%.
Example Embodiment
[0043] Example 3
[0044] 15g of pre-treated full-fat soybean meal was added to 1L of reverse micelle solution with AOT concentration of 80g / L, and 2.0% Alcalase alkaline protease was added at the same time to control the condition W 0 20. KCl concentration is 0.10mol / L, pH is adjusted to 7.0, extraction temperature is 45°C, and extraction is performed for 2 hours. The protein pre-extraction rate was 73.8%.
[0045] Add an equal volume of 0.8mol / L KCl-phosphate buffer solution, adjust the pH value to 8.0, oscillate in a shaker at a speed of 250r / min or more for 1.5h, then centrifuge for 20min, static layering to obtain the upper layer mixed oil, the lower layer protein aqueous solution. The protein post-extraction rate is 82.8%. The oil extraction rate is 87.5%.
[0046] Overall, the protein extraction rate was 61.1%, and the oil extraction rate was 86.0%. Adding Alcalase alkaline protease extraction, the degree of protein hydrolysis is about 4%, and the extraction rate is improve...
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