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Scutellaria teniana Hand tissue culture method

A technology of tissue culture and Scutellaria baicalensis, which is applied to plant cells, etc., can solve the problems of no guarantee of pollution-free, serious diseases and insect pests, and excessive use of pesticides, etc., and achieve the effect of many seedling roots, strong seedling roots, and high induction rate

Inactive Publication Date: 2009-01-28
CHUXIONG NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

For a long time, in the cultivation of Dayao scutellaria baicalensis, there have been problems such as self-propagation and self-propagation in the cultivation of Dayao scutellaria baicalensis, resulting in species degradation, serious pests and diseases, more pesticide use, and no pollution-free guarantee.

Method used

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  • Scutellaria teniana Hand tissue culture method
  • Scutellaria teniana Hand tissue culture method
  • Scutellaria teniana Hand tissue culture method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Scutellaria baicalensis Georgi was harvested from the Chuxiong Xingsheng Pharmaceutical Company’s drug garden. In order to reduce the pollution rate of the initial culture, it was harvested and planted in flower pots and placed in the laboratory. It was sprayed with 3% carbendazim every day for one week. Pre-culture. After that, follow the steps below to cultivate Dayao Scutellaria baicalensis tissue:

[0026] ①Take the stem tip of Scutellaria baicalensis Georgi as explants, after cleaning and disinfection, cut 0.2 cm from the top of the stem tip; or take the stem segments as explants, after cleaning and disinfection, cut into small stem segments of 0.5 cm, each segment Bring 1 true leaf; clean and disinfect the stem tip or stem section, soak it in a detergent solution for 5 minutes, rinse it in tap water for 5 minutes, soak it in 70% alcohol for 10 seconds, and soak it in 5% sodium hypochlorite for 15 minutes. Rinse with water 8 times.

[0027] ②Inoculate the stem tip or s...

Embodiment 2

[0032] Scutellaria baicalensis Georgi was harvested and planted in flower pots, placed in a laboratory, and sprayed with 3% carbendazim every day for a week of pre-culture. Then follow the steps below to cultivate Dayao Scutellaria baicalensis Georgi tissue:

[0033] ①Take the stem tip of Scutellaria baicalensis Georgi as explants. After cleaning and disinfection, cut the top 0.3 cm of the stem tip; or take the stem segments as explants, after cleaning and disinfection, cut into small stem segments of 1.5 cm, each segment Bring 2 true leaves; to clean and disinfect the stem tip or stem section, soak in a detergent solution for 5 minutes, rinse in tap water for 5 minutes, soak in 70% alcohol for 30 seconds, and soak in 10% sodium hypochlorite for 7 minutes. Wash with water 10 times;

[0034] ②Inoculate the stem tip or small stem segment on MS+BA 0.5mg / L+NAA 0.5mg / L medium, and cultivate it in the dark for a week to form callus;

[0035] ③Inoculate the callus or small stems of Scute...

Embodiment 3

[0039] Scutellaria baicalensis Georgi was harvested and planted in flower pots, placed in a laboratory, and sprayed with 3% carbendazim every day for a week of pre-culture. Then follow the steps below to cultivate Dayao Scutellaria baicalensis Georgi tissue:

[0040] ①Take the stem tip of Scutellaria baicalensis Georgi as explants. After cleaning and disinfection, cut 0.3 cm from the top of the stem tip; or take the stem segments as explants, and cut into 1 cm small stem segments after cleaning and disinfection. Bring 2 true leaves; to clean and disinfect the stem tip or stem segment, soak in a detergent solution for 5 minutes, rinse with tap water for 5 minutes, soak in 70% alcohol for 20 seconds, and soak in 8% sodium hypochlorite for 10 minutes. Rinse with water 9 times;

[0041] ②Inoculate the stem tip or small stem segment on MS+BA 0.25mg / L+NAA 0.25mg / L medium, and cultivate it in the dark for a week to form callus;

[0042] ③Inoculate the callus or small stems of Scutellaria...

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Abstract

The invention relates to a method for the culturing of scutellaria linn tissue; the method comprises the following steps: the stem tip of the scutellaria linn is adopted as explant, and after washed and disinfected, 0.2 to 0.3 cm of the top of the stem tip is cut; or the stem segment is taken as the explant, and after washed and disinfected, the stem segment is cut into 0.5 to 1.5cm of small segments and each segment is provided with one to two pieces of euphyllas; the stem tip or the stem segment is inoculated to culture medium of MS + 0.05 to 0.5mg / L of BA+0.05 to 0.5mg / L of NAA and dark culture is carried out to form the callus; the callus or the small stem segment is inoculated to the culture medium of MS + 0.5 to 3.0mg / L of BA+0.05 to 0.5mg / L of NAA and illumination culture is carried out to form the adventitious bud; the rootless seedling formed by the adventitious bud is inoculated to the culture medium of 1 / 2 MS+0.5 to 1.0mg / L of NAA to carry out culture till the tube seedling grows roots; the invention can induce the scutellaria linn adventitious bud to form and regenerate plants so as to expand the reproduction coefficient and culture the scutellaria linn which eliminates the disease and virus and has rejuvenation and good varieties by a manual method.

Description

Technical field [0001] The invention belongs to the technical field of plant regeneration through tissue culture technology, and is mainly a tissue culture method of Dayao Scutellaria produced in Dayao County, Chuxiong Prefecture, Yunnan. Background technique [0002] Dayao Scutellaria (Scutellaria.teniana Hand.Mazz) is a Yi medicine with local characteristics in Chuxiong Prefecture, Yunnan Province. The name of the Yi medicine is credit card Qi, which is one of the important raw materials of Yi medicine pharmaceutical industry. Taking root as medicine, cold in nature, bitter taste. It can eliminate heat and dampness, purify fire and detoxify, stop bleeding, and relieve fetus. It is used for chest tightness, nausea, damp heat, fullness of heat and diarrhea, diarrhea, jaundice, cough with lung heat, high fever and polydipsia, blood-heat vomiting, carbuncle swelling and sore poison, and fetal movement. Modern pharmacological experiments have proved that it has antibacterial, antipy...

Claims

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Application Information

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IPC IPC(8): C12N5/04
Inventor 邱璐范树国
Owner CHUXIONG NORMAL UNIV
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