Combination technique of organic blue dye for labeling the sentinel node and fluorescence imaging

Abstract

The invention relates to a combined technology for marking an organic blue dye of a sentinel lymph node and fluorescence imaging. The fluorescence given out by the organic blue dye is displayed by a fluorescence imaging device by the illumination of excitation light of the fluorescence imaging device with a wavelength not less than 570nm; wherein, the organic blue dye is one from methylene blue, Evans blue, isosulfan blue and patent blue or a compound thereof. The combined technology has the advantages of: overcoming the defect of needing to cut a larger opening on a skin for finding out a blue stain lymph node when using an organic dye to dye and position in the normal method for carrying out location of the sentinel lymph node of a tumor in clinical practice, and the sentinel lymph node can be located without an operation, thus reducing the pain of a patient. The combined technology has non-radioactivity, is convenient, fast and accurate and is a method which has excellent clinical application value.

Description

technical field [0001] The invention belongs to the field of biomedicine, and relates to a combination technology of organic blue dye and fluorescence imaging, in particular to a combination technology of organic blue dye for marking sentinel lymph nodes using methylene blue and the like as dyes and fluorescence imaging. Background technique [0002] The sentinel lymph node refers to the first station lymph node from the lymphatic drainage area of ​​the tumor. If there is tumor metastasis in the sentinel lymph node, the cancer cells are likely to have spread. At this time, the operation needs to be expanded. On the contrary, the scope of the operation can be reduced. The status of sentinel lymph nodes is an important basis for diagnosing tumor spread and determining treatment options. Compared with traditional lymph node dissection, sentinel lymph node biopsy can avoid unnecessary expansion of surgery and reduce patient pain. It is a major breakthrough in tumor surgery. [0...

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However, quantum dots contain toxic heavy metals such as cadmium (Cd)

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