Traditional Chinese medicine polysaccharide extract for suppressing lung cancer A549 cell proliferation and preparation method thereof
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A technology for polysaccharide extraction and cell proliferation, which is used in drug combinations, anti-tumor drugs, pharmaceutical formulations, etc.
Inactive Publication Date: 2009-03-25
NANJING UNIVERSITY OF TRADITIONAL CHINESE MEDICINE
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[0003] The treatment of tumors including lung cancer has always been a hot spot in clinical medical research and new drug development. However, due to the lack of specificity of traditional radiotherapy and chemotherapy, it often brings relatively large toxic and side effect...
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Embodiment 1
[0053] Example 1: Anti-lung cancer A549 cell polysaccharide extract and its preparation method
[0058] Take 10g of winter melon seeds, 12g of Pinellia chinensis, 10g of Dannanxing, 12g of mountain twigs, 10g of cat's claw, 10g of golden buckwheat, 10g of Houttuynia cordata, 15g of agrimony, 12g of sumac, 15g of safflower, 10g of sand ginseng, jade Bamboo 10g, Ophiopogon japonicus 10g, add 15 times the amount of water and decoct for 0.5 hour, filter and collect the extract, concentrate to obtain the whole prescription extract I (0.5g crude drug / ml).
[0059] 2. Preparation of polysaccharide crud...
Embodiment 2
[0063] Example 2: Anti-lung cancer A549 cell polysaccharide extract and its preparation method
[0068] Take 5g of winter melon seeds, 20g of Pinellia chinensis, 5g of Dannanxing, 10g of mountain twigs, 5g of cat's claw, 5g of golden buckwheat, 5g of Houttuynia cordata, 10g of agrimony, 20g of sumac, 10g of safflower, 5g of sand ginseng, jade Take 15g of bamboo and 15g of Ophiopogon japonicus, add 10 times the amount of water to decoct for 0.5 hour, filter and collect the extract, concentrate to obtain the whole prescription extract I (0.5g crude drug / ml).
[0069] 2. Preparation of polysaccharide crude extract I...
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Abstract
The invention discloses a Chinese traditional medicine amylase extractive for resisting lung cancer A549 cell proliferation, which is composed of the following materials by certain weight ratio: ternate buttercup root, cymose buckwheat rhizome, herba houttuyniae, agrimony, Chinese waxgourd seed, scarlet begonia, brazilwood, safflower, prepared pinellia tuber, arisaema cum bile, root of straight ladybell, polygonatum; the preparing method is that: firstly, the above 13 materials are added with water to be decocted and filtered so as to take the filtered liquid 1 out; and then the filtered liquid 1 is condensed to 0.5g medicine/ml; and then the condensed extracting liquid 1 is evenly mixed with ethanol and placed tranquilly and centrifuged, and the amylase gross extractive 2 is obtained through purifying the deposited ethanol with water; and finally the amylase gross extractive 2 is added with water to dissolve by heating and is extracted by equivalent chloroform-n-butanol; and then the liquid is strongly shaken and placed tranquilly for delamination so as to remove the water layer and the jello in the organic layer; the upper layer water can be condensed to 10g medicine/ml and can be dried in vacuum at 60 DEG C so as to obtain the amylase extractive; indicated by the experiment result of pharmacodynamics, the Chinese traditional medicine extractive has obvious effects on resisting the lung cancer A549 cell proliferation.
Description
technical field [0001] The invention relates to the technical field of traditional Chinese medicine, in particular to a polysaccharide extract of traditional Chinese medicine for resisting proliferation of lung cancer A549 cells and a preparation method thereof. Background technique [0002] Lung cancer (lung cancer) originates from bronchial mucosa and alveolar epithelial cells. It is one of the most common malignant tumors in the world today. Due to the rapid development of production, further deterioration of environmental pollution, and the prevalence of bad habits such as smoking, its incidence is on the rise in our country [1，2] . According to the forecast of the World Health Organization (WHO), by 2025, the number of new lung cancer deaths in my country will exceed 1 million each year, and the number of patients will rank first in the world. [3] . [0003] The treatment of tumors including lung cancer has always been a hot spot in clinical medical research and new ...
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IPC IPC(8): A61K36/898A61P35/00
Inventor 叶丽红尹莲张旭孙怡阳美平周仲瑛
Owner NANJING UNIVERSITY OF TRADITIONAL CHINESE MEDICINE
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