Method for preparing polyhydroxy taxone and paclitaxel

Abstract

The invention relates to a method for preparing polyhydroxy taxane and paclitaxel, which pertains to the manufacturing method of natural medical chemical substances or drug raw materials, aims at the tight source of paclitaxel, and selectively converts the multi-acetyl taxane with high abundance in the alcohol extract of yew plant material or cell culture into corresponding polyhydroxy taxane while separating the paclitaxel. The invention comprises the steps of resin column separation, alumina chromatographic column separation, hydrolysis reaction, the solid phase extraction of polyhydroxy taxane, the elution of polyhydroxy taxane, the purification of paclitaxel and the purification of polyhydroxy taxane, thus obtaining the paclitaxel with the purity approaching to 99.5 percent and the polyhydroxy taxane with the purity being more than 98 percent. The invention selectively converts the multi-acetyl taxane with high abundance into corresponding polyhydroxy taxane, produces raw materials which can be used in the pharmaceuticals industry, significantly reduces the loss of taxane compounds in the raw materials and improves the availability of biological resources.

Description

A method for preparing polyhydroxytaxane and paclitaxel technical field The invention belongs to the development of natural products or the production method of pharmaceutical raw materials, and specifically relates to the method of transforming and preparing polyhydroxytaxane and synchronously isolating paclitaxel by using yew plant material or alcoholic extract of cell culture as raw material. Background technique Paclitaxel (taxol) is a natural anti-tumor drug with significant curative effect. It also has a good application prospect in the treatment of AID-related Kaposi's Sarcoma (Kaposi's Sarcoma). Taxotere, and the third-generation paclitaxel 14-β-hydroxy deacetylbaccatin (ortataxel), which has entered the clinical phase II, have the same mother nucleus structure, and the difference is only in the individual substituent groups on the mother nucleus. For taxane compounds. Paclitaxel is a secondary metabolite of diterpenoids produced by Taxus chinensis. It has a compl...

AI Technical Summary

Problems solved by technology

In these methods, polyacetyltaxanes are usually removed as "impurities", resulting in a great waste of valuable natural resources

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