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Methods of using adipose tissue-derived cells in augmenting autologous fat transfer

A fat tissue and tissue technology, applied in the direction of bone/connective tissue cells, tissue culture, animal cells, etc., can solve the problems of limited self-renewal ability and inability to transport long-term products of fat cells

Inactive Publication Date: 2009-04-22
MACROPORE BIOSURGERY INC
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The problem associated with these measures is that the measure may only bring about a component of adipose tissue (adipocytes), while new blood vessels (angiogenesis) ar...

Method used

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  • Methods of using adipose tissue-derived cells in augmenting autologous fat transfer
  • Methods of using adipose tissue-derived cells in augmenting autologous fat transfer
  • Methods of using adipose tissue-derived cells in augmenting autologous fat transfer

Examples

Experimental program
Comparison scheme
Effect test

example 1

[0180] Example 1: Expression of angiogenic growth factor-VEGF by ADC

[0181] Vascular endothelial growth factor (VEGF) is one of the important regulators of angiogenesis (Nagy et al., 2003; Folkman 1995). Placental growth factor, another member of the VEGF family, plays a similar role in angiogenesis and arteriogenesis. Specifically, transplantation of wild-type (P1???GF+ / +) cells into P1GF-isolated mice restored the ability to allow rapid recovery from hindlimb ischemia (Scholz et al., 2003).

[0182] Assuming that angiogenesis and arteriogenesis are important for the revascularization process, the expression of P1GF and VEGF by the regenerative cells of the present invention was examined using an ELISA assay (R & D System, Minneapolis, MN) using Adipose-derived regenerative cells from three donors. One donor had a history of hyperglycemia and type 2 diabetes (a condition highly associated with microvascular and macrovascular disease). Renewable cells from each donor we...

example 2

[0185] Example 2: ADCs comprise cell populations involved in angiogenesis

[0186] Endothelial cells and their precursors, endothelial progenitor cells (EPCs), are known to be involved in blood vessel formation. To determine whether EPCs are within adipose-derived regenerative cells, human adipose-derived regenerative cells were evaluated for EPC cell surface markers such as CD-34.

[0187] ADCs were isolated by enzymatic digestion of human subcutaneous adipose tissue. ADC (100 microliters) was incubated in phosphate saline buffer (PBS) containing 0.2% fetal bovine serum (FBS), and incubated at 4 degrees Celsius for 20 to 30 minutes to allow fluorescent labeling Antibodies directed against human endothelial markers CD-31 (marker of differentiated endothelial cells) and CD-34 (EPC marker) and human ABCG2 (ATP-binding cassette transporter) selectively expressed on pluripotent cells. After washing, cells were analyzed on a FACSAria sorter (Beckton Dickenson-Immunocytometry). T...

example 3

[0190] Example 3: In Vitro Development of Vascular Structures in ADCs

[0191]A technique-approved assay for angiogenesis is that endothelial cells grown on a trophoblast of fibroblasts develop into a complex network of CD-31-positive tubes reminiscent of a nascent capillary network (Donovan et al. , 2001) test. Since adipose-derived regenerative cells contain endothelial cells, EPCs, and other stromal cell precursors, we tested the ability of these regenerative cells to form capillary-like structures in the absence of trophoblasts. Regenerative cells derived from the inguinal fat pad of normal mice developed capillary networks two weeks after culture (Fig. 18A). Remarkably, regenerative cells from hyperglycemic mice with type 1 diabetes induced with streptozotocin (STZ) formed capillaries 8 weeks after STZ administration like those formed by cells from normal mice An equivalent capillary network like the network (Fig. 18B).

[0192] In subsequent studies, adipose-derived r...

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Abstract

Methods of treating patients for conditions such as breast augmentation, soft tissue defects, and urinary incontinence, are described. The methods include removing adipose tissue from a patient, processing a portion of the adipose tissue to obtain a substantially isolated population of regenerative cells, mixing the regenerative cells with another portion of adipose tissue to form a composition, and administering the composition to the patient from which the adipose tissue are removed.

Description

[0001] related application [0002] This application is a continuation-in-part of U.S. Application No. 10 / 316,127, filed December 9, 2002, entitled "SYSTEMS AND METHODS FOR TREATING PATIENTS WITH PROCESSEDLIPOASPIRATE CELLS," which claims filing on December 7, 2001 Priority of U.S. Provisional Application No. 60 / 338,856. This application also claims priority to US Provisional Application No. 60 / 479,418, filed June 18, 2003, and entitled "METHODS OF USING ADIPOSE TISSUE DERIVED CELLS INAUGMENTING AUTOLOGOUS FAT TRANSFER." The contents of all aforementioned applications are expressly incorporated herein by reference. Background of the invention [0003] 1. Technical field [0004] The present invention relates generally to cells from adipose tissue, and more particularly, to adipose-derived regenerative cells (e.g., stem cells and / or progenitor cells), methods of using adipose-derived regenerative cells, adipose-derived regenerative cells comprising Compositions and systems ...

Claims

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Application Information

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IPC IPC(8): A61L31/00A61K35/12C12N5/08A61K35/28A61K35/44C12N5/0775
CPCC12N5/0667A61K35/44C12M47/04A61K35/28A61P13/00A61P37/06A61P43/00A61K48/00C12N5/0602
Inventor M·H·赫德里克J·K·弗拉塞尔
Owner MACROPORE BIOSURGERY INC
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