Method and system for quantitative hemoglobin determination

A hemoglobin, scheduled time technology, applied in the field of analysis, can solve problems affecting absorption differences

Inactive Publication Date: 2009-05-20
HEMOQUE AB
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, there are other factors that affect this difference in absorption

Method used

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  • Method and system for quantitative hemoglobin determination
  • Method and system for quantitative hemoglobin determination
  • Method and system for quantitative hemoglobin determination

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Embodiment Construction

[0050] will now see figure 1 The hemoglobin determination method according to the present invention is described. Step 1: First fill a disposable capillary tube with an unaltered whole blood sample. A sample to be analyzed is thus obtained. Step 2: Delayed analysis of the blood sample. This can be done by setting the instrument used to perform the analysis to resume the analysis after a predetermined time period has elapsed after the cuvette has been placed in the instrument holder. This delay will allow motion in the sample to quiet down, thereby eliminating the effect of scattered light from the red blood cells. Then, step 3: performing a first absorption measurement on the sample at wavelengths in the range 490-520 nm. Step 4: Further perform a second absorption measurement on the sample. The second absorption measurement is performed at wavelengths in the range 650-1200 nm. The second absorbance measurement is chosen such that the difference in absorbance between the...

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Abstract

A method for quantitative hemoglobin determination in undiluted, unhemolyzed whole blood comprises: acquiring a sample of unaltered whole blood into a capillary cuvette, presenting said cuvette to a set-up for an absorption measurement, delaying absorption measurement for a determined period of time, performing a first absorption measurement at a first wavelength in the range 490-520 nm directly on the sample in the cuvette, further conducting a second absorption measurement at a second wavelength different from the first wavelength and at which the absorption is substantially smaller than at the first wavelength, and processing results of the first and second absorption measurements to determine the concentration of hemo- globin in the sample. A system for implementing the method is also disclosed.

Description

technical field [0001] The present invention relates to a method of analysis, and a system for performing such an analysis. In particular, the present invention relates to methods for determining hemoglobin in unaltered whole blood and systems useful for such determinations. Background technique [0002] A disposable cuvette for sampling a liquid, mixing the sample with a reagent and performing optical analysis directly on the sample mixed with the reagent is disclosed as early as US Patent 4,088,448. This known cuvette has several advantages, such as: it simplifies the sampling process, reduces the number of vessels, and significantly increases the accuracy of the analysis by making the analysis process independent of the operator's technique performing the analysis. A test tube structure based on the same principle with improved flow characteristics is disclosed in US Patent 5,674,457. [0003] Disposable cuvettes developed according to these patents are currently widely...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/31G01N33/487G01N33/49
CPCG01N21/274G01N21/314G01N33/49
Inventor J·彼得松
Owner HEMOQUE AB
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