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Primer, application and kit for detecting polymorphic site of sulfonylurea receptor 1 gene

A polymorphic locus and gene technology, which can be used in the determination/examination of microorganisms, biochemical equipment and methods, etc., can solve the problems of individual differences, affect the efficacy of drugs, affect the occurrence of diseases, etc., to control blood sugar and reduce medical costs. , the effect of reducing toxic side effects

Active Publication Date: 2012-02-08
深圳泰乐德医疗有限公司
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AI Technical Summary

Problems solved by technology

Genetic background may not only affect the occurrence of diseases, but also individual differences in drug efficacy

Method used

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  • Primer, application and kit for detecting polymorphic site of sulfonylurea receptor 1 gene
  • Primer, application and kit for detecting polymorphic site of sulfonylurea receptor 1 gene

Examples

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Embodiment 1

[0030] Example 1 Detection of SUR1 gene T1369G polymorphism site genotype by PCR method

[0031] 1. Sample preparation

[0032] Twelve samples of EDTA anticoagulated blood were taken as sample sources, and samples were prepared according to the following steps.

[0033] 1. In a 1.5ml centrifuge tube, add 100μl of EDTA anticoagulated blood specimen

[0034] 2. Add 300 μl red blood cell lysate and incubate at 37°C for 20 minutes.

[0035] 3. Centrifuge and take the precipitate

[0036] 4. Add 100 μl leukocyte lysate, resuspend the pellet, and incubate at 37°C for 15 minutes

[0037] 5. Add 35 μl of protein precipitation solution and shake at high speed for 90 seconds.

[0038] 6. Centrifuge, take the supernatant, transfer all the supernatant into a 1.5ml centrifuge tube filled with 100μl isopropanol, and shake gently back and forth

[0039] 10 times until white flocs appear; centrifuge at 15000g for 90 seconds, and a white precipitate can be seen at the bottom of the centri...

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Abstract

The invention provides a specific primer for detecting the T1369G polymorphic site (rs757110) genotype of the SUR1 gene by a PCR method, and the specific primer is used in the preparation of reagents for detecting the T1369G polymorphic site of the SUR1 gene by PCR amplification of biological samples The application in the invention, the SUR1 gene T1369G polymorphic site detection kit containing specific primers and the application of the detection kit to predict the effect of sulfonylurea drugs. Sulfonylurea drugs are selected from glibenclamide, glibenclamide, glibenclamide, glibenclamide, glimepiride, glibenclamide, glibenclamide, glibenclamide, glibenclamide , glipizide, gliclazide, glipizide, and gliquidone, preferably gliclazide or glimepiride.

Description

technical field [0001] The invention relates to a specific primer for detecting the polymorphic site genotype of the sulfonylurea receptor gene by a PCR method, especially a specific primer for the T1369G (rs757110) polymorphic site of the sulfonylurea receptor 1 gene, And the use of the specific primer in preparing a reagent for detecting the T1369G polymorphic site of the sulfonylurea receptor 1 gene by PCR amplification of biological samples, and the T1369G polymorphic site of the sulfonylurea receptor 1 gene comprising the specific primer The point detection kit and the use of the kit to predict the effect of sulfonylurea drugs belong to the field of medical biotechnology. Background technique [0002] Sulfonylurea antidiabetic drugs are insulin secretagogues that lower fasting and postprandial blood glucose. For most patients with newly diagnosed type 2 diabetes, sulfonylurea antidiabetic drugs can reduce fasting blood sugar by 50-80 mg / dl and glycosylated hemoglobin (...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/68
Inventor 徐希平王燕王玉张彦明谢爱武于多邢厚恂
Owner 深圳泰乐德医疗有限公司
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