Detection method of traditional Chinese medicine composition capable of nourishing the heart to calm the mind
A detection method and a technique for nourishing the mind and soothing the mind, which can be used in drug combinations, pharmaceutical formulations, measuring devices, etc., can solve problems such as human harm, drug-induced enteritis, poisoning, etc., and achieve the effect of safety and high efficacy.
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experiment example 1
[0037] Experimental example 1. The preferred experiment of preparation process conditions and technical parameters of Chinese medicine composition of the present invention
[0038] (1) Process research
[0039] 1. Inspection of extraction process:
[0040]Commonly used extraction methods for licorice include water extraction and alcohol extraction. Experiments show that the main factor affecting the extraction efficiency in water extraction is the solvent volume, followed by extraction time and pH value of the solution. The extraction methods of Ganoderma lucidum include alcohol extraction and water extraction. Among them, the water extraction method has the highest polysaccharide content, which is about 5 times higher than that extracted with 70% ethanol. According to this, the experiment was carried out: 18 times, 22 times and 26 times of water were drawn up respectively for extraction to determine the appropriate solvent volume. In addition, the crushing degree of medicin...
experiment example 2
[0076] Experimental example 2. the TLC identification of Radix Glycyrrhizae in the pharmaceutical preparation of the present invention
[0077] (1) The investigation of the reflux extraction time during the preparation of the test solution
[0078] Get the preparation of the present invention equivalent to 8.0 g of raw medicinal materials, put it in a conical flask, add 50 ml of water, and ultrasonically filter, the filtrate passes through a macroporous adsorption resin column (1.5 cm in inner diameter, 15 cm long, D101), and elutes with 100 ml of 10% ethanol solution , discard the eluate, then add 50ml of 70% methanol solution to elute, collect the eluate, evaporate to dryness in a water bath, add 20ml of n-butanol to the residue to dissolve, shake and extract three times with ammonia test solution, the first 20ml, the second 2. Combine 10ml of ammonia test solution each time for three times, add hydrochloric acid to acidify, extract by shaking with n-butanol twice, 20ml each...
experiment example 3
[0094] Experimental example 3. Screening of assay methods
[0095] In order to improve the quality standard of the preparation, we determined the content of Junyao nacre powder in the prescription to improve the controllability of its quality.
[0096] 1. Reagents and reagents
[0097] Mettler Electronic Analytical Balance Hydrochloric Acid: AR Beijing Chemical Plant
[0098]Sodium Hydroxide: AR Beijing Chemical Plant Calcium Carbonate: AR Beijing Chemical Plant
[0099] 2. Establishment of the method
[0100] The preparation of need testing solution: get 20 tablets of the present invention, remove film coat, grind finely, accurately weigh 1.5g, put in 10ml centrifuge tube with stopper, add distilled water 5ml, shake to dissolve, centrifuge for 10 minutes, leave standstill , discard the upper solution, then add 5ml of distilled water, and operate 3 times in the same way; move the precipitate into a 250ml volumetric flask, add 10ml of hydrochloric acid solution (1→2ml) in po...
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