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Rhodococcus strain for degrading 3-nitrotoluene, as well as preparation method and use

A technology of nitrotoluene and rhodococcus, applied in the field of microorganisms, to achieve the effect of simple preparation method and convenient operation

Inactive Publication Date: 2012-06-27
WUHAN INST OF VIROLOGY CHINESE ACADEMY OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

At present, there is no report that purely cultured microorganisms can completely degrade 3-nitrotoluene compounds at home and abroad.

Method used

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  • Rhodococcus strain for degrading 3-nitrotoluene, as well as preparation method and use
  • Rhodococcus strain for degrading 3-nitrotoluene, as well as preparation method and use
  • Rhodococcus strain for degrading 3-nitrotoluene, as well as preparation method and use

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Embodiment Construction

[0024] The invention provides a preparation method and application of Rhodococcus ZWL3NT strain, which will be further described below with reference to the embodiments and accompanying drawings.

[0025] 1. Preparation method of Rhodococcus strain ZWL3NT

[0026] 1. Preparation of inorganic salt medium:

[0027] The ingredient is Na 2 HPO 4 ·12H 2 0 14.3g, KH 2 PO 4 3.0g, MnSO 4 ·H 2 O 0.28mg, FeSO 4 ·7H 2 O0.3mg, MgSO4 0.06mg, CaCl 2 1mg, CuSO 4 0.05mg, ZnSO 4 0.05mg and H 3 BO 3 0.05mg, make up to 1000ml with double distilled water, adjust pH to 7.0, 121°C, autoclave at 15 lbs for 30 minutes.

[0028] 2. Enrichment of degrading bacteria:

[0029] Take samples from the soil of Shandong Huayang Pesticide Company, add 5-20 grams of soil to 100 ml of inorganic salt medium to prepare a suspension, and then take 5-10 ml of soil suspension and inoculate 100 ml of 1mM 3-nitrotoluene In the inorganic salt medium, the constant temperature is 30 ℃, and the shaker is...

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Abstract

The invention discloses a Rhodococcus strain ZWL3NT CCTCC NO: M 208206. The preparation method of the strain includes formulation of an inorganic salt substrate, strain concentration, separation and degradation of bacteria, wherein the inorganic salt substrate contains Na2HPO4.12H2O, KH2PO4, MnSO4.H2O, FeSO4.7H2O and other components; the bacterial concentration includes: formulating the soil sample and inorganic salt substrate into a suspending liquid, inoculating the suspending liquid in a certain volume of inoculating amount onto a fresh inorganic salt medium containing 3-nitrotoluene, shaking table culturing at constant temperature until obtaining the accumulation culture object with significant degradation performance; the separation adopts a plating technique to separate and purify the culture in the LB medium. The bacterial strain provided by the invention can be used to degrade the 3-nitrotoluene with the degradation rate of 99 percent, and the preparation method is simple and easy to operate.

Description

technical field [0001] The invention relates to the field of microorganisms, in particular to a Rhodococcus strain capable of degrading 3-nitrotoluene and a preparation method and application thereof. Rhodococcus sp. ZWL3NT (Rhodococcus sp. ZWL3NT) was deposited by the China Center for Type Culture Collection (Address: School of Life Sciences, Wuhan University, Luojia Mountain, Wuchang, Wuhan City, 430072), the deposit date is November 10, 2008, and the deposit number is CCTCC NO: M 208206. Background technique [0002] Nitrotoluene compounds include three isomers, namely: 3-nitrotoluene (3-nitrotoluene, 3NT), 2-nitrotoluene (2-nitrotoluene, 2NT), 4-nitrotoluene (4-nitrotoluene, 4NT). As raw materials and intermediates, it is widely used in the production and manufacture of various dyes, pesticides and medical drugs. They have many ways to enter the environment and become pollutants in the production process. The harm of nitrotoluene to the human body is similar to that ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A62D3/00
Inventor 王淑君刘虹周宁一
Owner WUHAN INST OF VIROLOGY CHINESE ACADEMY OF SCI
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