Method for measuring content of Bletilla striata medicinal materials

A determination method and content technology, applied in the field of content determination of P.

Inactive Publication Date: 2009-08-12
正安县绿野科技产业有限责任公司 +1
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Problems solved by technology

However, as a raw material in the field of medicine, the content of its active ingredients must reach a certain standard, otherwise, it will greatly affect its application value in the field of

Method used

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  • Method for measuring content of Bletilla striata medicinal materials
  • Method for measuring content of Bletilla striata medicinal materials
  • Method for measuring content of Bletilla striata medicinal materials

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Experimental program
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Embodiment Construction

[0058] Determination of bis[4-(β-D-glucopyranoseoxy)benzyl]-2-isobutylmalate (militarine) in Baiji medicinal materials according to high performance liquid chromatography (Appendix VID of "Chinese Pharmacopoeia") content.

[0059] Instruments and reagents: Agilent 1100 HPLC system, including quaternary gradient pump, variable wavelength detector, diode array detector, autosampler, 7725i manual injection valve and Chemstation workstation; TCQ-250 ultrasonic cleaner; Column, Symmetry C 18 (5μm, 150mm×4.6mm, Waters company); Bis[4-(β-D-glucopyranose oxygen) benzyl]-2-isobutyl malate (militarine) reference substance (Guizhou traditional Chinese medicine, national medicine Research and Development Center, the purity is greater than 98%); Methanol is chromatographically pure, water is double distilled water, and other reagents are analytically pure.

[0060] (1) Chromatographic conditions and system suitability test: Octadecylsilane bonded silica gel is used as filler, methanol: w...

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Abstract

The invention discloses a method for measuring the content of common bletilla pseudobulb medical materials. The method adopts bis(4-(Beta-D-pyranoglucose oxygen) benzyl)-2-isobutylmalate as a comparison product and uses high performance liquid chromatography to measure the content of the bis(4-(Beta-D-pyranoglucose oxygen) benzyl)-2-isobutylmalate of the common bletilla pseudobulb medical materials. The method is convenient, fast and accurate and provides good standard for controlling the quality of the common bletilla pseudobulb medical materials.

Description

technical field [0001] The invention relates to a quality control method for Radix Radix and medicinal materials, in particular to a method for determining the content of Radix Radix Radix and medicinal materials. Background technique [0002] Bletilla striata (Thunb.) Reichb.f. is a dry tuber of the orchid plant Bletilla striata (Thunb.) Reichb.f., bitter, sweet, astringent, slightly cold in nature, and has the functions of astringent, hemostasis, swelling and granulation. It is a commonly used herbal medicine. Chinese medicinal materials, used in Chinese patent medicines such as mink pancreas anti-crack ointment and Gushangling spray. With the deepening of modern pharmacological research, it is found that it has obvious inhibitory effect on Mycobacterium tuberculosis and tumor cells. It has been found clinically that Baiji medicinal materials also have good curative effects on common diseases such as internal and external hemorrhage, burns and scalds. However, as a raw m...

Claims

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Application Information

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IPC IPC(8): A61K36/898G01N30/36
Inventor 王爱民王永林兰燕宇何迅李勇军周树勇张秀玥罗光琼
Owner 正安县绿野科技产业有限责任公司
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