Eureka AIR delivers breakthrough ideas for toughest innovation challenges, trusted by R&D personnel around the world.

Composition for diagnosing amyloid-related disease

A technology of amyloid and correlation, applied in preparations for in vivo experiments, in vivo radioactive preparations, organic chemistry, etc., can solve problems such as uninvolved diseases

Inactive Publication Date: 2011-07-20
NAGASAKI UNIVERSITY
View PDF5 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Therefore, these reported compounds have not yet entered into practical application for the diagnosis of diseases associated with amyloid accumulation

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Composition for diagnosing amyloid-related disease
  • Composition for diagnosing amyloid-related disease
  • Composition for diagnosing amyloid-related disease

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0114] experimental method

[0115] Reagents and Instruments

[0116] Amyloid β protein (human, 1-42) [TFA type] was purchased from Peptide Institute, Inc., and special grade reagents were used as other reagents. Measured using Varian Gemini 300 and tetramethylsilane as internal standard 1 H-NMR.

[0117] (1) Synthesis of chalcone derivatives containing aromatic heterocycles

[0118] Synthesis of (E)-3-(4-(dimethylamino)phenyl)-1-(5-iodothiophen-2-yl)prop-2-en-1-one (1)

[0119] 256 mg (1 mmol) of 5-acetyl-2-iodothiophene and 149 mg (1 mmol) of 4-dimethylaminobenzaldehyde were dissolved in ethanol (20 mL), and 10% aqueous potassium hydroxide solution (30 mL) was added to the mixture under ice-cooling middle. React at room temperature for 6 h, then add 30 mL of purified water, and filter the precipitated crystals by suction. The crystals were washed with 50% aqueous ethanol and dried to obtain target compound 1. Yield 238mg (62.1% yield). 1 H NMR (300MHz, CDCl 3 )δ...

Embodiment 2

[0137] experimental method

[0138] Reagents and Instruments

[0139] As radioactive iodine-125 ( 125 I) Iodine-125 (3.7 GBq / mL) manufactured by MP Biomedicals, Inc. was used. At a flow rate of 1.0 mL / min, Cosmosil 5C manufactured by Nacalai Tesque Inc. was used 18 -AR-II column (4.6×150 mm) and ultrapure water: acetonitrile = 3:7 as eluent, reversed-phase HPLC. Using Varian Gemini 300 with tetramethylsilane as internal standard, measure 1 HNMR. Mass spectrometry was performed using a JEOL IMS-DX300. Using 12PLC plate 20 x 20cm manufactured by MERCK - Silica Gel 60F 254 , 2 mm for preparative TLC. Amyloid β protein (human, 1-42) was purchased from Peptide Institute, Inc., and special grade reagents were used as other reagents.

[0140] (1) Synthesis of chalcone derivatives

[0141] Synthesis of 4-bromo-4'-nitrochalcone (1)

[0142] 4-Nitroacetophenone (1.67g, 10.1mmol) and 4-bromobenzaldehyde (1.86g, 10.0mmol) were dissolved in ethanol (10mL), and 10% potassium h...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

There is provided a composition comprising a compound represented by general formula (I), wherein R 1 represents a 5-iodothiophen-2-yl group or the like, and R 2 represents a 4-dimethylaminophenyl group or the like. This composition is useful for diagnosis of an amyloid-related disease such as Alzheimer's disease because the compound has high binding specificity to amyloid protein, high permeability through the blood-brain barrier, and a property of being rapidly eliminated from sites other than senile plaques in the brain.

Description

technical field [0001] The present invention relates to a composition for diagnosing amyloid-related diseases such as Alzheimer's disease, a method for screening therapeutic or preventive drugs for amyloid-related diseases using the above-mentioned composition, and evaluating A method for treating or preventing an amyloid-related disease. Background technique [0002] With the rapid aging of the population in recent years, an increase in the number of patients suffering from dementia-related diseases including Alzheimer's disease (AD) has become a serious social problem. Currently, Hasegawa Scale, ADAS and MMSE are available methods for clinical diagnosis of AD. All of these methods quantitatively assess reduced cognitive function in AD-suspect individuals and are commonly used. In addition, image diagnostic methods (MRI, CT, etc.) are used supplementarily. However, these diagnostic methods are insufficient to definitively diagnose AD, and development of senile plaques an...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): A61K51/00C07D213/61C07D233/64C07D277/24C07D307/42C07D333/28C07D333/36C07D409/06
Inventor 中山守雄原武卫小野正博
Owner NAGASAKI UNIVERSITY
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com