A kind of preparation method and application of nucleic acid aptamer modified chitosan nanofiber

Abstract

The invention discloses a method for preparing chitosan nanofibers modified by nucleic acid aptamers. The preparation method comprises the following steps: (1) preparing a chitosan solution; (2) preparing chitosan nanofibers; (3) Chemical immobilization: take the nucleic acid aptamer solution, add EDC / NHS solution to activate, then add the chitosan nanofibers obtained in step (2), and shake and react at room temperature; The reaction mixture was subjected to solid-liquid separation to obtain the nucleic acid aptamer-modified chitosan nanofibers. The nucleic acid aptamer-modified chitosan nanofibers of the present invention are suitable for the highly selective separation and enrichment of trace or ultra-trace mycotoxins or other substances in food and the environment, and have high selectivity and high extraction capacity. The advantages of large size, high stability and simple preparation.

Description

technical field [0001] The invention belongs to the technical field of chemical analysis and testing, and relates to dispersed solid-phase microextraction technology, in particular to a method for preparing a nucleic acid aptamer-modified chitosan nanofiber adsorbent. Highly selective separation and enrichment of components such as trace mycotoxins. Background technique [0002] Due to the limitations of the state of the components to be measured, the interference of coexisting components, or the low sensitivity of the determination method, most chemical detection and analysis methods require effective and reasonable pretreatment of the sample in advance to achieve enrichment and purification. Effect. However, compared with the rapid development of instrumental analysis technology, the progress of sample pretreatment technology is relatively slow. Currently commonly used sample pretreatment techniques such as liquid-liquid extraction and column chromatography generally hav...

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Problems solved by technology

[0007] Aiming at the problems of poor selectivity and low immobilization rate of nucleic acid aptamers in the existing solid phase extraction technology, the present invention combines the characteristics of nucleic acid aptamers with high affinity, strong specificity, and good compatibility with biological samples with chitosan nanofibers Combining the advantages of large specific surface area and easy chemical modification, a nucleic acid aptamer-modified chitosan nanofiber was developed for the separation and enrichment of trace or ultra-trace components in complex samples. Liquid chromatography, which can realize high selectivity and high sensitivity analysis and detection

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