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Method for separating and purifying resveratrol in polygonum cuspidatum

A technology for separation and purification of resveratrol, applied in chemical instruments and methods, preparation of organic compounds, organic chemistry, etc., can solve the problems of low product yield, cumbersome operation, high cost, etc., and achieve high yield and simple operation , the effect of short cycle

Inactive Publication Date: 2013-10-30
SUN YAT SEN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Above-mentioned prior art operation is loaded down with trivial details, and used equipment is many, and cost is high and product yield is low

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Grind 500g of dried knotweed medicinal material, use a solution of acetone / water 6 / 4 (v / v) as the extraction solvent, the amount of extraction liquid is 1:10 (g / v) according to the material / liquid, and ultrasonically extract twice, each After 35 minutes, filter and collect the filtrate, adjust the pH of the filtrate to weak alkaline (pH 7.5-9) with caustic alkali solution, add a certain volume of ethyl acetate, so that the ratio of ethyl acetate / acetone / water in the system is 5 / 6 / 4 (v / v / v), fully shake, stand at 35°C for 40 minutes, collect the upper phase solution, concentrate under reduced pressure to recover the solvent, to a small volume (about 5ml remaining), let stand, and precipitate solids. The resveratrol product is obtained; the product adopts the single-point fixed-point method, and is analyzed and identified by TLC scanning technology. The purity of resveratrol is above 85%; the extraction rate is determined by the resveratrol content in Polygonum cuspidatum...

Embodiment 2

[0026] Grind 500g of dried knotweed medicinal material, use a solution of acetone / water at 7:3 (v / v) as the extraction solvent, and use a dosing / liquid ratio of 1:10 (g / v) for the extract, and ultrasonically extract twice, each time 35min, filter, collect the filtrate, adjust the pH of the filtrate to weak alkaline (pH7.5-9) with caustic alkali solution, add a certain volume of dichloromethane, so that the ratio of dichloromethane / acetone / water in the system is 5 / 7 / 3(v / v / v), fully shake, stand at 35°C for 40 minutes, collect the lower phase solution, concentrate under reduced pressure and recover the solvent to a small volume (about 5ml remaining), let it stand, and precipitate the solid to obtain Resveratrol products; the product adopts the single-point fixed-point method and is analyzed and identified by thin-layer scanning technology. The purity of resveratrol is above 85%. The extraction rate is determined by the content of resveratrol in Polygonum cuspidatum. Veratrol (t...

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Abstract

The invention discloses a method for separating and purifying resveratrol in polygonum cuspidatum. The method provided by the invention purifies the resveratrol in the polygonum cuspidatum by constructing an alkali ternary two-phase liquid-liquid extracting system; the whole procedure is composed of simple operations including extraction, primary extraction, primary crystallization and the like; the method has the advantages of simplicity in operation, low cost, short purifying period and the like, and does not need an expensive and complicated chromatographic separation device. The resveratrol product prepared by the method has high purity, does not contain acid anthraquinones coexisted component, has no resveratrol loss during a purification process, and has an extracting rate which is higher than that of the traditional method.

Description

technical field [0001] The invention relates to the technical field of medicine separation and purification, in particular to a method for separating and purifying resveratrol in Polygonum cuspidatum. Background technique [0002] Resveratrol is hailed as another new green anti-cancer drug after paclitaxel, which can significantly inhibit cancer cells, lower blood lipids, prevent and treat cardiovascular diseases, resist oxidation, enhance immunity, and delay aging. It is widely used in industries such as pharmaceuticals, food, health care products and cosmetics. [0003] In recent years, health foods with resveratrol as the active ingredient emerge in endlessly: Japan adds resveratrol to various wines as a food additive, and prepares low-alcohol, high-resveratrol alcohol that has a good preventive effect on cardiovascular diseases. New health-care table wine; Paradise her Resveratrol and Natrol resveratrol from Canada, Tiens Live Health from Tianjin Tiens Group, and probio...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07C39/21C07C37/70
Inventor 王军向玲丽尹胜李春燕姚美村杨润潮兰晶周少耿郑晶
Owner SUN YAT SEN UNIV
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