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Separation technology for two-aqueous-phase extraction of spirulina phycocyanin through polyvinyl ether ampicillin

A technology for polyvinyl ether ampicillin and phycocyanin, which is applied in the field of chemical separation engineering, can solve the problems of high price, cumbersome process, poor selectivity for separating phycocyanin, etc., and achieves low production cost, high extraction selectivity, and the solution to utilization of and the effect of the hypervalue problem

Inactive Publication Date: 2017-05-31
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The reported patented technology of two-phase extraction and separation of phycocyanin for the preparation of algae phycocyanin, such as Chinese patent CN103880950A, uses expensive ionic liquids to form a polymer-water phase, which increases production costs. CN102993297A, CN101891809A, etc. use commercially available PEG constitutes a polymer-water phase, and the selectivity of separating phycocyanin is poor. It needs to be combined with other extraction methods such as salting out to obtain high-purity products, and the process is cumbersome.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] (1) Preparation of spirulina broken wall serum

[0018] Take the dry powder of Spirulina platensis produced in Cibaiian Biological Engineering Co., Ltd., Dongtai City, Jiangsu Province, use deionized water as the extraction agent, and the solid-liquid ratio of algae powder to deionized water is 1:30, and freeze at -20 ° C for 4 Hours later, thaw at 37°C, repeat freezing and thawing for 5 times, centrifuge the thawed cyanobacteria broken wall liquid at 6000 rpm for 10 minutes, take the supernatant as the broken wall supernatant, and the protein concentration in the supernatant is 1.0mg / ml, phycocyanin purity A 620 / A 280 It is 0.752mg / ml.

[0019] (2) Preparation of brominated polyvinyl ether

[0020] In the round bottom flask of 500ml, put into 100g polyethylene glycol PEG-1000 (purchased from Hai'an Petrochemical Plant), the average molecular weight of PEG-1000 is 1000g every mole, load 35g phosphorus tribromide (purchased in constant pressure funnel) From Huai’an ...

Embodiment 2

[0028] (1) Preparation of spirulina wall breaking liquid

[0029] Take fresh spirulina produced in Jiangsu Dafengci Century Biotechnology Co., Ltd., use 100mmol / L phosphate buffer as the extractant, and the solid-liquid ratio of spirulina to deionized water is 1:150, and freeze at -15°C After 4 hours, it was thawed at 37°C and repeated freezing and thawing 5 times. The thawed cyanobacteria broken wall solution was centrifuged at 6000 rpm for 10 minutes with a centrifuge, and the supernatant was taken as the broken wall supernatant, the protein concentration in the supernatant was 3.6mg / ml, and the purity of phycocyanin was A 620 / A 280 is 0.65.

[0030] (2) Preparation of brominated polyvinyl ether

[0031] In the round bottom flask of 1000ml, put into 100g polyethylene glycol PEG-3000 (purchased from Hai'an Petrochemical Plant), the average molecular weight of PEG-3000 is 3000g every mole, in the constant pressure funnel, load 45g phosphorus tribromide (purchased From Hua...

Embodiment 3

[0039] (1) Preparation of spirulina wall breaking liquid

[0040] Take the spirulina powder produced in the spirulina base in Chenghaiyuan, Lijiang, Yunnan, use 200mmol / L phosphate buffer as the extractant, the solid-liquid ratio of spirulina to deionized water is 1:150, and freeze at -20°C for 4 hours Finally, thaw at 37°C, and repeat the freezing and thawing process 6 times. The thawed cyanobacteria wall-broken liquid was centrifuged at 6000 rpm for 10 minutes with a centrifuge, and the supernatant was taken as the wall-broken supernatant, and the purity of phycocyanin in the supernatant was A 620 / A 280 is 0.62, and the protein concentration of the broken wall fluid is 7.6mg / ml.

[0041] (2) Preparation of brominated polyvinyl ether

[0042] In the round-bottomed flask of 2000ml, put into 100g polyethylene glycol PEG-4000 (purchased from Hai'an Petrochemical Plant), the average molecular weight of PEG-4000 is 4000g every mole, load 25g phosphorus tribromide ( Purchased ...

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Abstract

The invention discloses a separation technology for two-aqueous-phase extraction of spirulina phycocyanin through polyvinyl ether ampicillin, and belongs to the technical field of chemical separation engineering. The separation technology comprises the following steps: preparation of the polyvinyl ether ampicillin; two-phase extraction of the phycocyanin; obtaining of phycocyanin powder after concentration and freeze drying, wherein the phycocyanin recycling rate can reach 75 to 95 percent. The method is simple; wall breaking fluid of spirulina is enriched and separated by directly using the polyvinyl ether ampicillin, so that the steps in a purification process are simplified; the selectivity is high; the spectral purity of the phycocyanin obtained by separation is up to A620 / A280 greater than 3.5. Fresh spirulina or dry spirulina powder can be used as a raw material, so that a technological method for realizing high-value-scale use of spirulina resources is provided.

Description

technical field [0001] The invention provides a preparation method of polyvinyl ether ampicillin, which is used for extracting phycocyanin from spirulina wall breaking liquid. The invention belongs to the technical field of chemical separation engineering. Background technique [0002] Spirulina phycocyanin is present in the phycobilisomes of Spirulina. Phycobilisomes are supramolecular light-harvesting pigment complexes unique to red and cyanobacteria. Phycocyanin is bright blue in color and bright in color. It is the first choice of pure natural pigment for food, high-end eye shadow and lipstick. Phycocyanin can also regulate and synthesize a variety of important enzymes required by human metabolism, has obvious anti-oxidation and anti-inflammatory effects, regulates the human immune system, and enhances the function of the immune system. High-purity phycocyanin has strong fluorescence, and it is made of pure natural fluorescent reagents, which are used in research fiel...

Claims

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Application Information

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IPC IPC(8): C07K14/795C07K1/34C07K1/14
CPCC07K14/795
Inventor 王峰
Owner JIANGNAN UNIV
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