Earthnut acetyl coenzyme A carboxylase carboxyltransferase beta subunit gene and encoding protein and cloning method thereof

A carboxylase carboxyl, acetyl coenzyme technology, applied in the field of genetic engineering, to achieve the effect of increasing oil content
CN101550421AInactive Publication Date: 2009-10-07SHANDONG PEANUT RES INST
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
SHANDONG PEANUT RES INST
Publication Date
2009-10-07
Estimated Expiration
Not applicable · inactive patent

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Abstract

The present invention discloses an earthnut acetyl coenzyme A carboxylase carboxyltransferase beta subunit gene and an encoding protein and a cloning method thereof. The gene sequence is composed of a nucleotide sequence from 92th to 1570th bits of SEQ ID No.1; the protein encoded by the gene has an amino acid sequence represented by the SEQ ID No.2 in a sequence table. The nucleotide sequence of the earthnut acetyl coenzyme A carboxylase carboxyltransferase beta subunit (AhCT beta) gene and the encoding protein sequence and the gene cloning method of the invention may be used for improving the earthnut quality and the earthnut cultivation widely, especially a field where the earthnut is used for developing and using oil crops.
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Description

technical field

[0001] The invention relates to the technical field of genetic engineering, in particular to peanut acetyl-CoA carboxylase carboxyltransferase beta subunit gene and its encoded protein and a cloning method. Background technique

[0002] Acetyl-CoA Carboxylase (ACCase) is a biotin-containing enzyme that catalyzes the carboxylation of acetyl-CoA to form malonyl-CoA in vivo, providing substrates for the synthesis of fatty acids and many secondary metabolites (Konishi T, Shinohara K, Yamada K. Acetyl-CoA carboxylase in higher plants: most plants other than gramineae have both the prokaryotic and the eukaryotic forms of this enzyme [J]. Plant Cell Physiol, 1996, 37(1): 17-122 .). There are 2 types of ACCase in organisms. A type of ACCase called Homomeric, also known as multifunctional or eukaryotic, exists in the cytosol of animals, yeast, algae and plants, and has a biotin containing subunit with a relative molecular weight of 220~260 . The other is heterogen...

Claims

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