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A kind of efficient breeding method of Eurasian seedless grape

A seedless grape, high-efficiency technology, applied in horticultural methods, botanical equipment and methods, plant cells, etc., can solve the problems of failing to achieve the purpose of cultivation, low breeding efficiency, low probability of seedless, etc., to increase the range of parental selection, The effect of improving the development rate of young embryos and accelerating the breeding process

Active Publication Date: 2011-12-14
HANGZHOU BLUE SKY LANDSCAPE CONSTR GROUP
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] 2. The zygotic embryos of seedless grapes are aborted during the development process and cannot develop into normal seeds. Therefore, conventional breeding can only use seedless varieties as male parents to crossbreed seedless grape varieties, but the probability of seedless grapes in the offspring is low ( 0~15.9%), low breeding efficiency
In terms of the sampling time of immature embryos, if the collection time is too early, the immature embryos are too small to survive; if the collection time is too late, the immature embryos have already died, and the purpose of cultivation cannot be achieved.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] Embodiment 1: a kind of high-efficiency breeding technology of Eurasian seedless grapes, specifically comprising the following steps: (1) conventional pollination and fertilization during the flowering period, picking young fruits 30 to 50 days after flowering, washing with running water under running water for 1hr, and ultra-clean workbench First, soak the young fruit with 70% alcohol for 1 minute, rinse with sterile water for 3 times; then soak with 0.1% mercuric chloride for 10 minutes, then rinse with sterile water for 4 to 5 times, (2) cut the fruit under aseptic conditions Ovules were removed from the berries and inoculated on embryo culture media containing different levels of plant growth regulators gibberellin acid (GA3), indole acetic acid (IAA), and 6-benzylaminoadenine (6-BA). save. (3) Conditions for embryo rescue: place the inoculated Erlenmeyer flask in a 5-8°C incubator for 60 days at low temperature, and then transfer it to a culture room for 30 days. ...

example 1

[0029] Example 1: Seedless white grapes are emasculated and bagged three days before the first flowering period. Choose a sunny morning (from 6:00 to 7:00) when the temperature is 20-30°C and there is no wind. When emasculating, remove the corolla and stamens together with pointed tweezers. It is necessary to remove the stamens and avoid damaging the stigma. In order to prevent self-fertilization caused by pollen falling on the stigma during emasculation, the stigma is washed with water after each emasculation, and bagged immediately after drying. More than 100 grains per ear. 100 ears of each variety were emasculated. For each variety, at least 20 vigorous plants should be selected as female parents. Post-hybridization is managed using conventional techniques.

[0030] Pick the young fruit 30-50 days after flowering, wash it under running water for 1 hour, soak the young fruit in 70% alcohol for 1 minute under aseptic conditions on the ultra-clean workbench, and rinse it...

example 2

[0036] Example 2: Excellent seedless grapes (super seedless, yellow grape) are emasculated and bagged three days before the first flowering period. Choose a sunny morning (from 6:00 to 7:00) when the temperature is 20-30°C and there is no wind. When emasculating, remove the corolla and stamens together with pointed tweezers. It is necessary to remove the stamens and avoid damaging the stigma. In order to prevent self-fertilization caused by pollen falling on the stigma during emasculation, the stigma is washed with water after each emasculation, and bagged immediately after drying. More than 100 grains per ear. 100 ears of each variety were emasculated. For each variety, at least 20 vigorous plants should be selected as female parents. Post-hybridization is managed using conventional techniques.

[0037] Pick the young fruit 30-50 days after flowering, wash it under running water for 1 hour, soak the young fruit in 70% alcohol for 1 minute under aseptic conditions on the ...

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Abstract

The invention relates to a high-efficiency breeding method for Eurasian seedless grapes. It is to pick young grape fruits 30 to 50 days after flowering of seedless grapes, take out ovules under aseptic conditions, and inoculate the embryos in plants containing different levels. Embryo rescue was performed on MS medium with the growth regulators gibberellic acid (GA3), indoleacetic acid (IAA), 6-benzylaminoadenine (6-BA). Using the present invention, the average embryo development rate is increased from 8.3 to 11.9% of the patent published by the background method to 84%, the germination rate reaches 75.33%, and the seedling rate reaches 74.17%, and the seedless grape embryo rescue method obtained by the application passes Improving the embryo development rate and germination rate of seedless grapes, increasing the seedling rate of offspring, can obtain more hybrid offspring more easily, increase the parental selection range of seedless grape hybrid breeding, and accelerate the breeding process of new seedless grape varieties. Improve seedless grape breeding efficiency.

Description

technical field [0001] The invention relates to a high-efficiency breeding method for Eurasian seedless grapes, belonging to the technical field of plant breeding. Background technique [0002] 1. Publication No. CN 101238791A, titled "A Method for Improving the Breeding Efficiency of Seedless Grapes", which is a medicament prepared by dissolving a plant growth inhibitor substance chlormequat or a cytokinin substance 6-BA 10-20 days before flowering, spray on flower spikes, pick grape fruit grains 40-50 days after flowering, take out ovules and carry out embryo rescue, and the embryo development rate of annuclei of the present invention is increased by 0-4.4%. to 8.3-11.9%; Moritani Seedless increased from 0.5-2.5% to 1.8-21.9%; Flame Seedless increased from 7.1-17.1% to 14.1-25.4%. [0003] 2. The zygotic embryos of seedless grapes are aborted during the development process and cannot develop into normal seeds. Therefore, conventional breeding can only use seedless varieti...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N5/04A01H4/00
Inventor 刘海琳
Owner HANGZHOU BLUE SKY LANDSCAPE CONSTR GROUP
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