Homocysteine dry chemical detection strip and preparation method thereof
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A technology of homocysteine and reagent strips, which is applied in the detection of homocysteine reagent strips and its manufacturing field, can solve the problems of undetected homocysteine, etc., and achieve good specificity, sensitivity, specificity, Simple operation effect
Inactive Publication Date: 2009-12-02
BEIJING HUAANFO BIOMEDICAL RES CENT +1
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At present, no dry chemical reagent strips for the detection of homocysteine have been found at home and abroad, including literature reports and invention patents
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Embodiment 1
[0027] Embodiment 1: the preparation method of homocysteine dry chemical test strip, it comprises the steps:
[0028] The composition of the specific test strips is attached figure 1 , 2 , 3.
[0029] 1) Prepare the carrier membrane 6 with the enzyme reagent adsorbed:
[0030] Prepare the buffer system, lyase, auxiliary enzyme, surfactant, reducing agent, protective agent, and stabilizer into a reaction solution, soak the above solution into the carrier film, and then dry the carrier film in a drying oven;
[0031] 2) Preparation of the carrier film 7 adsorbed with the developer:
[0032] The substrate and the activator are prepared into a chromogenic solution, the above solution is soaked into the carrier film, and then the carrier film is dried in a drying oven;
[0033]3) After the above-mentioned prepared carrier film is completely dried, according to the reaction sequence, the developer layer 7 is first adhered to the lower support layer 2, and then the enzyme reage...
Embodiment 2
[0040] Embodiment 2: the usage method of homocysteine dry chemical test strip
[0041] 1. Take out the reagent strips according to the required quantity and set aside;
[0042] 2. Take 30 microliters of peripheral blood / plasma / serum from the subject and add it directly to the sample hole 3;
[0043] 3. After reacting for 5-10 minutes, observe the color behind the transparent film at the lower end;
[0044] 4. Judgment of the result: if there is obvious blue, it is positive (more than 15uM / L); if it is colorless or light blue, it is negative (less than 15uM / L);
[0045] 5. Or use a reflectance spectrophotometer to measure. The reflectance spectrophotometer performs calculations with preset programs and prints the results.
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Abstract
The invention provides a homocysteine dry chemical detection strip and a preparation method thereof. The invention aims to provide a detection strip and a preparation method thereof for fast semiquantitatively/quantitatively detecting homocysteine. The strip is composed of an elongated upper support layer, an elongated lower support layer and test layers in the middle and is divided into a hand-held area and a test area. A diffusion layer, a filtration layer, an enzyme reagent layer and a colour development reagent layer are arranged in the test area from top to bottom. Various grid materials of synthetic fibre materials can be used to prepare the diffusion layer, various filterable materials for separating blood can be used to prepare the filtration layer, and various asymmetric synthetic membranes can be used to prepare the enzyme reagent layer and the colour development reagent layer. A loading hole is arranged in the upper support layer, a sample is sent from the loading pole to the diffusion layer, the sample then permeates into the filtration layer, the enzyme reagent layer and the colour development reagent layer, chemical reactions are performed in the enzyme reagent layer and the colour development reagent layer to change the colour, and the change of optical density in reaction process can be tested through a test pole of the lower support layer.
Description
technical field [0001] The invention relates to a reagent strip for detecting homocysteine and a manufacturing method thereof, belonging to the field of medical testing. Background technique [0002] Homocysteine (homocysteine, Hcy) is an intermediate product of methionine metabolism in the human body, and also participates in the transsulfuration pathway in vivo. Homocysteine is remethylated to methionine via the folate cycle by B12 in the presence of 5-methyltetrahydrofolate, and the subsequently produced 5,10-dimethyltetrahydrofolate is absorbed by methyltetrahydrofolate reductase (MTHFR ) reduced to 5-methyltetrahydrofolate. Homocysteine can also be remethylated to methionine by glycine trimester. Remethylation is completed in the liver and kidney. In the methionine cycle, methionine in food is converted into S-adenosylmethionine (SAM). SAM is used as a substrate for the methyl group donor of methyltransferase. S-adenosylhomocysteine (SAH) is the only produc...
Claims
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