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Electric spray ion source

An electrospray ion source, electrospray technology, applied in ion source/gun, parts of particle separator tube, material analysis by electromagnetic means, etc., can solve the problems of different spray voltage, low ionization ionization efficiency, etc. Achieving the effect of solving inefficiencies

Active Publication Date: 2009-12-09
SINOMS BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] The invention relates to an electrospray ion source, which is used to solve the problem of low ionization efficiency when using a fixed electrospray voltage for electrospray ionization of complex protein mixtures. Proteins with different compositions and conformations require different spray voltages.

Method used

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Examples

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Effect test

Embodiment 1

[0031] Such as figure 1 As shown, an electrospray ion source is used for mass spectrometry detection of proteins and other macromolecules. It includes a three-dimensional operating table and an electrospray device placed on a three-position operating platform. The electrospray device includes one end connected to the liquid chromatography fraction. , the connection assembly connected to the spray needle at the other end and the electrospray voltage applied to the electrospray device, the electrospray ion source further includes an oscillating voltage module for controlling the electrospray voltage, for generating an oscillating voltage;

[0032] The oscillating voltage module includes a high-voltage module provided with a low-voltage terminal and a high-voltage terminal,

[0033] The high voltage module includes a high voltage coil transformer 11 for converting low voltage into high voltage AC;

[0034] A voltage doubler 12 (HV Multiplier) connected to the output end of the ...

Embodiment 2

[0039] Such as image 3 , Figure 4 As shown, an electrospray ion source is used for mass spectrometry detection of proteins and other macromolecules. It includes a three-dimensional operating table and an electrospray device placed on a three-position operating platform. The electrospray device includes one end connected to the liquid chromatography fraction. , the connection assembly connected to the spray needle at the other end and the electrospray voltage applied to the electrospray device, the electrospray ion source further includes an oscillating voltage module for controlling the electrospray voltage, for generating an oscillating voltage;

[0040] The oscillating voltage module includes a high-voltage module provided with a low-voltage terminal and a high-voltage terminal,

[0041] The high voltage module includes a high voltage coil transformer 21 for converting low voltage into high voltage AC;

[0042] A voltage doubler 22 connected to the output end of the high...

Embodiment 3

[0047] Such as Image 6 , Figure 7 As shown, an electrospray ion source is used for mass spectrometry detection of proteins and other macromolecules. It includes a three-dimensional operating table and an electrospray device placed on a three-position operating platform. The electrospray device includes one end connected to the liquid chromatography fraction. , the connection assembly connected to the spray needle at the other end and the electrospray voltage applied to the electrospray device, the electrospray ion source further includes an oscillating voltage module for controlling the electrospray voltage, for generating an oscillating voltage;

[0048] The oscillating voltage module includes a high-voltage module provided with a low-voltage terminal and a high-voltage terminal,

[0049] The high voltage module includes a high voltage coil transformer 31 for converting low voltage into high voltage AC;

[0050] A voltage doubler 32 connected to the output end of the high...

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Abstract

The invention provides an electric spray ion source used for a mass spectrum. An oscillating voltage module is used for controlling electric spray voltage so that the electric spray voltage linearly rises to high voltage from low voltage in a short time and then declines to the low voltage from a high voltage gradient, and the voltage is oscillated and scanned back and forth, so different voltages are suitable for different macromolecular substances to be ionized, and the ionization efficiency of a macromolecular mixture is improved. The oscillating voltage module is integrated on a circuit board, is quite easy to assemble and debug, and is convenient to use so that the electric spray mass spectrum is more suitable for the detection of complex biological macromolecules such as proteomics and the like; the mass spectrum identification rate is improved; and the detection sensitivity is improved.

Description

technical field [0001] The invention relates to an electrospray ion source for mass spectrometry, and more specifically relates to the electrospray ion source technology for ionizing macromolecules such as proteins by using an oscillating voltage module to control the electrospray voltage. Background technique [0002] Electrospray technology is a soft ionization technology, which was first proposed by Fenn et al. It has been widely used since then. Electrospray applies an electric field to generate charged droplets, which then deliver analyte ions to a mass spectrometer for analysis by ion evaporation. This soft ionization technology is suitable for the ionization of biological macromolecules such as proteins, but the efficiency of this soft ionization method is relatively low, usually 1 / 10 4 , The stability of the spray also needs to be improved, and the tolerance to non-volatile salts is also relatively low. [0003] Therefore, many people have done research on improvi...

Claims

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Application Information

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IPC IPC(8): H01J49/10G01N27/62
Inventor 徐国宾聂磊清江杨芃源
Owner SINOMS BIOTECH CO LTD
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