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Streptococcus-pneumoniae-toxicity-reducing live vaccine

A technology for Streptococcus pneumoniae and viable bacteria, which is applied to antibacterial drugs, bacterial antigen components, etc., can solve the problems of high price, low coverage, and cannot be promoted, and achieves the effects of low cost, short cycle and simple manufacturing process.

Inactive Publication Date: 2011-11-09
CHONGQING MEDICAL UNIVERSITY
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  • Abstract
  • Description
  • Claims
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AI Technical Summary

Problems solved by technology

The vaccine can also prevent systemic infection and mucosal infection, and prevent colonization of the nasopharynx, thereby reducing the spread of bacteria in the community. It is composed of capsular polysaccharides combined with non-toxic diphtheria CRM197 protein. It is expensive and is under development. country cannot promote
In addition, there is capsular serotype switching during the application of this vaccine, and the included serotypes cover 65%-80% of the serotypes associated with invasive pneumococcal disease in young children in Western industrial countries, however, in many developing The coverage rate of the country may be low, and the duration is only 2-3 years

Method used

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  • Streptococcus-pneumoniae-toxicity-reducing live vaccine

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Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0024] 1. Construction of defective bacteria D39Δ1672

[0025] (1) Materials:

[0026] Bacterial DNA extraction kit was purchased from Shanghai Huashun Bioengineering Co., Ltd. rTaq enzyme, dNTPs, Buffer, MgCl for PCR 2 Bought from Dalian Bao Biological Technology Co., Ltd., the gel recovery kit was purchased from Shanghai Huashun Bioengineering Co., Ltd. Competent state stimulating peptide (CSP 1 ) was purchased from Jill Biochemical (Shanghai) Co., Ltd. and synthesized, and the PCR instrument was Gene cycleTM of Bio-Rad Company of the United States.

[0027] (2) Design and synthesis of primers;

[0028] Using Streptococcus pneumoniae D39 genomic DNA as a template, refer to its complete sequence (No. NC008533), and use primer premier5.0 to design primers. Primers were synthesized by TAKARA Company.

[0029] Up P1: Positive 5-TCCAAGGTTTCTGGGTCT-3

[0030] P2: trans 5-ATCAAACAAATTTTGGGCCCGG-CATTGTTCCGTCCGTATT-3

[0031] Down P3: Positive 5-AATTCTATGAGTCGCTGCCGACT-TTG...

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Abstract

The present invention relates to a streptococcus-pneumoniae-toxicity-reducing live vaccine used for preventing streptococcus-pneumoniae infection. The vaccine is the composition of D39delta1672 streptococcus pneumoniae strains which contain streptococcus pneumoniae SPD-1672 and have gene function deficiency, can effectively prevent the streptococcus-pneumoniae infection, has strong safety and good protection effect, and is easy for large-scale production and potential for generalization.

Description

technical field [0001] The invention relates to the technical field of biomedicine, in particular to a live attenuated Streptococcus pneumoniae vaccine for preventing Streptococcus pneumoniae infection. Background technique [0002] Streptococcus pneumoniae (S.pn) is one of the leading causes of morbidity and mortality in children and adults worldwide. It can spread non-invasively and persistently from its mucosal habitat in the nasopharynx to the lungs causing pneumonia, blood causing sepsis, cerebrospinal fluid causing meningitis, or other sites causing disease (otitis media, sinusitis, tracheitis). At present, the total number of deaths from Streptococcus pneumoniae infection in the world is as high as 3 to 5 million people every year, most of whom are children, the elderly and people with low immune function. In addition, due to the increasing use of antibiotics, the drug resistance rate of Streptococcus pneumoniae continues to increase. 44% of the clinically isolated ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K39/09A61P31/04
Inventor 尹一兵张雪梅吴凯峰姚润庞丹
Owner CHONGQING MEDICAL UNIVERSITY
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