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Method of sonatic embryo generation and plant regeneration of aleuritopteris argentea

A technology of somatic embryos and silver powder back ferns, which is applied in the field of plant reproduction to achieve the effects of high multiplication multiple, good stability and fast reproduction speed

Inactive Publication Date: 2010-02-03
BEIJING FORESTRY UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, due to the huge difference in the characteristics of different species, the original species, medium, culture conditions and specific results obtained for induction and proliferation are very different.

Method used

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  • Method of sonatic embryo generation and plant regeneration of aleuritopteris argentea

Examples

Experimental program
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Effect test

Embodiment 1

[0038] Obtaining regenerated plants of P. argentina by inducing somatic embryos in liquid suspension culture:

[0039] (1) Spore disinfection and germination: Take about 2 mg of mature spores and place them in a 2 mL centrifuge tube, add 1.5 mL of 5% NaClO aqueous solution, centrifuge at 3500 r / min for 1 min, pour off the supernatant, add sterile water and wash for 4 to 5 Finally, transfer the spores into a 10mL centrifuge tube and dilute with sterile water. The density of the spore suspension is about 3×10 3 When cells / mL (counted by a hemocytometer), inoculate the suspension evenly on MS solid medium with a dropper to induce germination. The culture temperature is (24±2)°C, the light intensity is 2400 lux, and the light time is 16 h / d. After about 2 weeks, the spores germinate and continue to culture until prothallus are formed.

[0040] (2) Prothallus subculture: the prothallus grown in the step (1) is inoculated into MS liquid medium, the container is a 150ml Erlenmeyer f...

Embodiment 2

[0047] Obtaining regenerated plants of P. argentina by inducing somatic embryos in solid culture:

[0048] (1) Spore disinfection and germination: the same as Example 1, but the light intensity is 2000lux.

[0049] (2) Prothallus subculture: the prothallus develops into a mature prothallus after being continuously cultured in the solid medium in step (1) for 3 weeks, and the culture conditions are the same as in step 1).

[0050] (3) Callus induction and proliferation: Cut the mature prothallus mass in step (2) into 1cm×1cm agglomerates, inoculate them into MS solid medium, add KT2.0mg / l, 2,4- D2.0mg / l, other culture conditions are the same as the previous step. Callus pieces were induced after 6-8 weeks of culture.

[0051] (4) Induction and proliferation of embryogenic callus: Subculture the above callus into MS liquid medium, add 1.0 mg / l of 6-BA and 500 mg / l of sodium dihydrogen phosphate. After about 4 weeks of culture, the callus transformed into embryogenic callus ma...

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Abstract

The invention relates to a method for breeding aleuritopteris argentea rapidly and abundantly by the using induction of sonatic embryo and plant regeneration, comprising the following steps: 1) induction and proliferation of callus; 2) induction and proliferation of embryonic callus; 3) induction of sonatic embryo; and 4) induction of regenerated plant. The invention solves the problems of low natural seedling survival rate, long seedling cycle and low reproduction coefficient of aleuritopteris argentea in the prior art; compared with the conventional method which utilizes spores for reproduction, the invention has the advantages of low investment, high reproductive rate, high increase multiples and good stability, can abundantly produce and retain regenerated plant with excellent female parent properties; the seedling survival rate is increased by 10-15 times; in addition, the method of the invention provides a good asexual reproduction experimental system for the improvement of aleuritopteris argentea and for the screening of transgenes receptor and mutants.

Description

technical field [0001] The invention relates to the field of plant propagation, in particular to a method for rapidly propagating fern argentina. Background technique [0002] The promotion and application of native foliage plants is one of the hot research fields of landscaping in China. Foliage plants can be roughly divided into two types, one is colorful leaf plants, and the other is green leaf plants with simple colors and graceful postures. Among the latter, ferns are unique because of their simplicity, elegance, lightness and harmonious lines. They are known as the extraordinary masters in the plant kingdom and are deeply loved by people. [0003] Although my country has abundant resources of wild ferns, only a dozen or so species of ferns are found in the flower market as small potted plants or accessories for flower arrangements, and they are far from being scaled up. This is related to my country's relatively weak reproductive technology. Ferns are the lowest leve...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 董丽张启翔黄笛冯玉兰程堂仁
Owner BEIJING FORESTRY UNIVERSITY
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