Traditional Chinese biological preparation for preventing and treating livestock diarrhea and preparation method thereof

A technology for biological preparations and livestock and poultry diarrhea, which is applied in the direction of pharmaceutical formulations, drug combinations, and medical preparations containing active ingredients, etc., to achieve the effects of easy absorption, sufficient drug efficacy, and mild reaction conditions

Active Publication Date: 2010-03-10
BEIJING DABEINONG ANIMAL HEALTH TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there are no research reports at home and abroad on the microbial transfor

Method used

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  • Traditional Chinese biological preparation for preventing and treating livestock diarrhea and preparation method thereof
  • Traditional Chinese biological preparation for preventing and treating livestock diarrhea and preparation method thereof

Examples

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Example Embodiment

[0029] Example 1 Preparation of traditional Chinese medicine biological preparation of the present invention

[0030] Composition of Pulsatilla powder: 60 parts of Pulsatilla, 50 parts of Cork, 60 parts of Qin Pi, 30 parts of Coptis

[0031] Preparation:

[0032] (1) Inoculate yeast strains into YPD medium, culture at 30℃, 180rpm shaker to produce first-grade seed liquid, when the content of viable bacteria reaches 1.0×10 8~9 When cfu / ml, transfer to the secondary seed liquid fermentation tank to expand the culture, the inoculum amount is 6%, the culture temperature is 30℃, the stirring speed is 220rpm, and the viable bacteria content of the secondary seed liquid reaches 1.0×10 8~9 When cfu / ml, stop the culture and reserve;

[0033] (2) Take the Chinese medicinal materials of the above formula, crush them into 120 mesh medicinal powder, add 10% of the weight of the Chinese medicine, 2% glucose, 0.5% urea, 1.0% K 2 HPO 4 , Add water to 50% water content, adjust the pH to 4, and steril...

Example Embodiment

[0037] Example 2 Preparation of traditional Chinese medicine biological preparation of the present invention

[0038] Composition of Pulsatilla powder: 80 parts of Pulsatilla, 60 parts of Cork, 80 parts of Qin Pi, 50 parts of Coptis

[0039] Preparation:

[0040] (1) Inoculate yeast strains into YPD medium, culture at 30℃, 180rpm shaker to produce first-grade seed liquid, when the content of viable bacteria reaches 1.0×10 8~9 When cfu / ml, transfer to the secondary seed liquid fermentation tank to expand the culture, the inoculum amount is 8%, the culture temperature is 30℃, the stirring speed is 220rpm, and the viable bacteria content of the secondary seed liquid reaches 1.0×10 8~9 When cfu / ml, stop the culture and reserve;

[0041] (2) Take the Chinese medicinal materials of the above formula and pulverize into 100 mesh medicinal materials, add 8% of the weight of the Chinese medicine, 3% glucose, 0.7% urea, 1.5% K 2 HPO 4 Add water to 60% water content, adjust the pH to 4.5, and st...

Example Embodiment

[0044] Example 3 Preparation of Traditional Chinese Medicine Biological Preparation of the Invention

[0045] Composition of Pulsatilla powder: 50 parts of Pulsatilla, 45 parts of Phellodendron, 50 parts of Qinpi, 45 parts of Coptis

[0046] Preparation:

[0047] (1) Inoculate yeast strains into YPD medium, culture at 29℃, 190rpm shaker to produce first-grade seed liquid, when the content of viable bacteria reaches 1.0×10 8~9 When cfu / ml, transfer to the secondary seed liquid fermentor to expand the culture, the inoculum amount is 5%, the culture temperature is 29℃, the stirring speed is 200rpm, and the viable bacteria content of the secondary seed liquid reaches 1.0×10 8~9 When cfu / ml, stop the culture and reserve;

[0048] (2) Take the Chinese medicinal materials of the above formula and crush them into fine powder of 90 mesh medicinal materials, add 9% of the weight of the Chinese medicine, 4% glucose, 0.8% urea, 0.7% K 2 HPO 4 , Add water to 55% water content, adjust pH to 5, and...

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Abstract

The invention discloses a traditional Chinese biological preparation for preventing and treating livestock diarrhea and a preparation method thereof. The traditional Chinese biological preparation isprepared by combining a traditional prescription and a modern microbiological transformation technology, utilizing the strong transformation capacity of microorganism and fermenting and metabolizing the traditional Chinese pulsatilla root. The preparation method comprises the following steps: crushing the Chinese pulsatilla root; adding bran, glucose, urea, inorganic salt and water as a fermentation medium; adjusting the pH value; adding a strain culture liquid with a certain proportion for fermenting after sterilization; adding ethanol into matured solid leavening for lixiviating, separating,filtering, concentrating and preparing various preparations. The traditional Chinese biological preparation is prepared by the Chinese pulsatilla root after being fermented and metabolized by yeast,and has greatly superior treatment effect to Chinese pulsatilla root medicinal powder and decoctum thereof for the livestock diarrhea.

Description

Technical field [0001] The invention relates to a traditional Chinese medicine biological preparation for preventing and treating diarrhea of ​​livestock and poultry and a preparation method thereof, and belongs to the field of veterinary medicine. Background technique [0002] Dysentery and diarrhea are common digestive tract diseases in veterinary clinics. Most of them are caused by Escherichia coli, Salmonella, cocci, etc. The incidence of various diseases in livestock and poultry is very high, and they can occur throughout the year. Veterinarians mainly use antibiotics or chemical drugs to treat livestock and poultry diarrhea in clinical practice. The treatment effect is not good, and the drug resistance of pathogenic bacteria is becoming more and more serious. The traditional Chinese medicines used to treat this disease include Zhili powder, Chulijing, Baitouweng powder, Wumei powder, etc. The dosage forms of these drugs are mainly traditional powders, with low technical con...

Claims

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Application Information

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IPC IPC(8): A61K36/064A61K36/756A61P1/12C12N1/16A23K1/16A23K10/12A23K10/30
Inventor 黄建茹莫云刘澜周德刚葛冰张俊儒葛代兴田玲邓利斌丛日刚
Owner BEIJING DABEINONG ANIMAL HEALTH TECH
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