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Artemisia scoparia extractive and production method and applications thereof

An extract and artemisia artemisiae, applied in the artemisia artemisiae extract and its production method and application field, can solve the problem that flavonoid components have not been officially reported, and achieve significant anti-influenza virus, reliable process, and stable total flavonoid content Effect

Inactive Publication Date: 2012-07-11
XINJIANG INST OF MATERIA MEDICA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0013] At present, the preparation of extracts from Artemisia spp. and its genus plants to develop antiviral drugs has not been reported at home and abroad. Macroporous resin technology and / or polyamide resins are used to separate and purify flavonoids from Artemisia spp. and its genus plants. There is no official report on the study of similar components

Method used

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  • Artemisia scoparia extractive and production method and applications thereof
  • Artemisia scoparia extractive and production method and applications thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0044] Example 1: add 8 to 10 times the amount of water to the aboveground part of Artemisia fragrans, boil and extract twice at 80°C to 100°C, each time for 2 hours to 3 hours, filter, combine the filtrates, and concentrate to one-third of the original volume , add 1 to 2 times the volume of the concentrated solution of absolute ethanol and place it for precipitation for 12 hours, filter the precipitate, adjust the pH value to 3 to 6 with dilute hydrochloric acid after the filtrate is concentrated, and put it on the AB-8 macroporous resin chromatography column , after all the sample solution is absorbed, rinse the column with 3 to 8 column volumes of deionized water, and then perform gradient elution with 50%, 70%, and 90% ethanol, and the amount of ethanol per concentration is 3 to 8. 8 chromatographic column volumes, the flow rate is 2 to 5 chromatographic column volumes per hour, collect the ethanol eluent, combine and concentrate at 60°C to 90°C, and then dry the concentra...

Embodiment 2

[0045] Example 2: Add 8 to 10 times the amount of water to the aboveground part of Artemisia marina, boil and extract twice at 80°C to 100°C, each time for 2 hours to 3 hours, filter, combine the filtrates, and concentrate to one-third of the original volume , add 1 to 2 times the volume of the concentrated solution of absolute ethanol and place it for precipitation for 12 hours, filter the precipitate, adjust the pH value to 3 to 6 with dilute hydrochloric acid after the filtrate is concentrated, and put it on the AB-8 macroporous resin chromatography column , after all the sample solution is absorbed, rinse the column with 3 to 8 column volumes of deionized water, and then perform gradient elution with 30%, 50%, and 70% ethanol, and the amount of ethanol per concentration is 3 to 8. 8 chromatographic column volumes, the flow rate is 2 to 5 chromatographic column volumes per hour, collect the ethanol eluent, combine and concentrate at 60°C to 90°C, and then dry the concentrate...

Embodiment 3

[0046] Example 3: add 8 to 10 times the amount of water to the aerial parts of Artemisia marina, boil and extract twice at 100°C, each time for 2 hours to 3 hours, filter, combine the filtrates, concentrate to one-third of the original volume, and add the concentrate 1 to 2 times the volume of absolute ethanol was left to settle for 12 hours, filtered to remove the precipitate, and the filtrate was concentrated and adjusted to a pH value of 3 to 6 with dilute hydrochloric acid, put on an AB-8 macroporous resin chromatography column, and waited for sample loading After the solution is completely absorbed, wash the column with 3 to 8 column volumes of deionized water, and then perform gradient elution with 10%, 30%, and 50% ethanol. The amount of ethanol per concentration is 3 to 8 column volumes. The column volume and the flow rate are 2 chromatographic column volumes per hour. The ethanol eluents are collected, combined and concentrated at 60°C to 90°C, and the concentrated sol...

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Abstract

The invention discloses an artemisia scoparia extractive and a production method and applications thereof. The artemisia scoparia extractive contains 25-75 percent of a flavonoid component; the production method of the artemisia scoparia extractive comprises the following steps: 1. crudely extracting the overground part or herba of the artemisia scoparia by water or ethanol; 2. adding anhydrous ethyl alcohol to the extracting solution after filtration and concentration, standing and precipitating, and filtering the sediment to obtain ethanol solution; 3. concentrating the obtained ethanol solution, adjusting the pH value, conducting adsorption on a macroporous resin and / or polyamide resin chromatographic column; 4. conducting gradient elution by 10%-90% ethanol, collecting the ethanol eluent and merging; and 5. concentrating the eluent, recovering the ethanol, and drying under reduced pressure, thus obtaining the required artemisia scoparia extractive. Proved by the experimental study, the artemisia scoparia extractive has remarkable effects on resisting influenza viruses, hepatitis B virus and human immunodeficiency virus, and safe oral drug administration, and can be used for preparing broad-spectrum antiviral drugs for treating influenza, hepatitis B and acquired immune deficiency syndrome.

Description

technical field [0001] The present invention relates to the technical field of extracts extracted from plants and their production methods and applications, and is the technical field of Artemisia marina extracts and their production methods and applications. Background technique [0002] Influenza is an acute respiratory infectious disease caused by influenza virus. Epidemiologically, it is characterized by high morbidity, rapid onset, strong infectivity and certain mortality (infants and young children, the elderly and infirm, immune or cardiopulmonary function Insufficient persons, often secondary bacterial infection after infection with influenza virus, especially easy to cause pneumonia, often life-threatening). About 500 million people in the world are infected with influenza virus every year, and an influenza pandemic will break out every 10-30 years, which will bring huge social burden and economic loss. It can be said that influenza is the biggest plague in human so...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K36/28A61P31/16A61P31/18A61P31/20
Inventor 黄华刘燕徐芳姚华贺金华王林林毛燕
Owner XINJIANG INST OF MATERIA MEDICA
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