Electrochemical receptor biosensor and application thereof

A biosensor and electrochemical technology, applied in the field of electrochemical receptor biosensors, achieves the effects of less sample consumption, easy large-scale promotion and application, and broad market prospects

Inactive Publication Date: 2010-03-17
SHANGHAI UNIV
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  • Electrochemical receptor biosensor and application thereof
  • Electrochemical receptor biosensor and application thereof
  • Electrochemical receptor biosensor and application thereof

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Embodiment Construction

[0045] 1. Recombinant EGFP-β 2 - Preparation of AR cell membrane: the present invention selects the recombinant EGFP-β cultured in the stage of induced expression for 30 h 2 -AR yeast cells, obtained by breaking and extracting recombinant EGFP-β by classical glass bead method 2 AR cell membrane, and against recombinant EGFPβ 2 -AR cell membrane was identified by morphological electron microscopy, fluorescence identification and radioligand binding analysis experiments. The results showed that: recombinant EGFP-β 2 -AR cell membrane not only has biological activity, but also complete biological function. For the negative control system, the preparation of unrecombined GS115 yeast cell membrane was obtained by the same method as above.

[0046] 2. Recombinant EGFP-β 2 - AR cell membrane modified working electrode preparation: take 50uL 1.0mg / mL recombinant EGFP-β 2 -AR cell membrane and 50uL 1.0mg / mL lecithin resuspension buffer were fully mixed in an eppndorf tube, then tu...

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Abstract

The invention relates to an electrochemical biologic receptor sensor taking a recombination EGFP-beta 2-AR cell membrane as a biosensor identification element and an application thereof in the detection of a beta 2-excitant. The electrochemical receptor biosensor comprises a working electrode, a counter electrode and a reference electrode. The electrochemical receptor biosensor is characterized inthat the working electrode adopts a gold electrode modified by the recombination EGFP-beta 2-AR cell membrane; the counter electrode adopts a platinum wire electrode; and the reference electrode adopts a saturated calomel electrode. The expression system established by the invention is successful, establishes a firm technical foundation for a beta 2 receptor-matcher detection system and also strongly prompts that the combination of the beta 2 receptor-matcher detection system and the electrochemical biosensor provides feasibility for searching and establishing the quick, sensitive and broad-spectrum detection of the beta 2-excitant.

Description

technical field [0001] The present invention relates to a new electrochemical receptor biosensor and its application, especially a recombinant EGFP-β 2 -AR cell membrane electrochemical bioreceptor sensor as biosensor recognition element and its role in detecting β 2 -Agonist application. technical background [0002] beta 2 - Adrenergic agonists (beta for short) 2 - Agonist, beta 2 - Stimulants, beta 2 - Ligand) is a class of hormone drugs with a large number of members, including Clenbuterol (Clenbuterol, commonly known as Clenbuterol), Salbutamol (Salbutamol), Ractopamine (Ractopamine) and dozens of other (commonly known as Clenbuterol) , and its mechanism of action is: β 2 - Ligand first binds to β 2 - Adrenergic receptors (abbreviated as β 2 -receptor), exerts physiological effects through the G protein-coupled cAMP signal transduction pathway, and is clinically used for the treatment of related diseases such as asthma and bronchospasm. Following the same mecha...

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Application Information

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IPC IPC(8): G01N27/26
Inventor 陈宇光彭可顺毛春鸿戴小峰
Owner SHANGHAI UNIV
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