Method for collecting and culturing ulva spores

Abstract

The invention discloses a method for collecting and culturing ulva spores, which comprises the following steps: placing mature ulva segments on a glass slide, dropwise adding seawater, irradiating with light with the illumination intensity of 3,500-4,200Lx to stimulate the ulva segments, collecting spores when discovering planospores are released and then culturing the planospores. In the invention, the mature ulva is irradiated by strong light so as to promote the planospores to release and meet the requirement for mass collection. The method for collecting and culturing the ulva spores has simple and practical operation, has low requirement on the mature degree of the ulva, not only stably and continuously provides a great deal of spores with normal growth and development capability for experiments, but also is convenient to observe the attachment and bourgeon status of the spores on large scale, thereby the provided method makes up the blank of the field of ulva spore collection and culture, establishes the technological base for large-scale manual culture and has wide application prospect in the aspects of alga culture, prevention test research, and the like.

Description

technical field

[0001] The invention belongs to the field of biotechnology, and relates to a method for collecting and cultivating Ulva spores. Background technique

[0002] According to the survey results of fouling organisms in various coastal sea areas of my country, it can be seen that large algae such as Ulva, Enteromorpha, and water cloud are the dominant species of algae fouling organisms that are common in autumn, winter, and spring. The attachment of algae will significantly increase the dynamic load effect of waves and currents on marine facilities, causing drift or even overturning; blocking the mesh holes of aquaculture cages, cages, seines, etc., affecting the exchange of water in the internal and external environment, reducing the internal environment. Dissolved oxygen hinders the normal growth and development of breeding objects.

[0003] Ulva lactuca is edible and medicinal. It mostly grows on rocks or bogs in middle and low tidal zones, and can also grow on...

Examples

Embodiment 1

[0023] The culture medium is natural seawater, and after the seawater is filtered, boiled and sterilized, and cooled to room temperature, it is placed in a high-temperature sterilized vessel for use.

[0024] The collected fresh Ulva was placed in sterilized seawater for temporary maintenance.

[0025] Take about 2 cm of yellow-green algae with brown edges on a glass slide, add a small amount of sterilized seawater dropwise, and place it on a Nikon MD-TS100 microscope stage.

[0026] The spore collection test was divided into three groups: group A, light intensity 3500Lx; group B, light intensity 500-3000Lx; group C, control group, indoor natural light irradiation. The light time is 10 minutes, and seawater is added dropwise in time to prevent evaporation from drying up the algae. Microscopic examination, use a pipette to absorb the released spores, that is, to absorb them with a pipette, and place them in a 50ml small beaker for later use. Repeat the above steps to continue...

Embodiment 2

[0029] The culture medium is natural seawater, and after the seawater is filtered, boiled and sterilized, and cooled to room temperature, it is placed in a high-temperature sterilized vessel for use.

[0030] The collected fresh Ulva was placed in sterilized seawater for temporary maintenance.

[0031] Take about 2 cm of yellow-green algae with brown edges on a glass slide, add a small amount of sterilized seawater dropwise, place it on a Nikon MD-TS100 microscope stage, and stimulate it for 5 minutes with a light intensity of 4000 Lx. Microscopic examination found that the zoospores were sucked up with a straw and placed in a 50ml small beaker for later use. Repeat the above steps to continue collecting spores 2 times. Replace with fresh algae fragments, and repeat the above steps to collect spores.

[0032] Take a small amount of spore liquid in a beaker and count it on a hemocytometer to determine the number of zoospores per milliliter of water, and dilute it with sterili...

Embodiment 3

[0037] The culture medium is natural seawater, and after the seawater is filtered, boiled and sterilized, and cooled to room temperature, it is placed in a high-temperature sterilized vessel for use.

[0038] The collected fresh Ulva was placed in sterilized seawater for temporary maintenance.

[0039] Take about 2 cm of yellow-green algae with brown edges on a glass slide, add a small amount of sterilized seawater dropwise, place it on a Nikon MD-TS100 microscope stage, and stimulate it for 3 minutes with a light intensity of 420Q Lx. Microscopic examination found that the release of zoospores was sucked up with a straw, and placed in a 50ml small beaker for later use. Repeat the above steps to continue collecting spores 2 times. Replace with fresh algae fragments, and repeat the above steps to collect spores.

[0040] Take a small amount of spore liquid in a beaker and count it on a hemocytometer to determine the number of zoospores per milliliter of water, and dilute it wit...

technical field

[0001] The invention belongs to the field of biotechnology, and relates to a method for collecting and cultivating Ulva spores. Background technique

[0002] According to the survey results of fouling organisms in various coastal sea areas of my country, it can be seen that large algae such as Ulva, Enteromorpha, and water cloud are the dominant species of algae fouling organisms that are common in autumn, winter, and spring. The attachment of algae will significantly increase the dynamic load effect of waves and currents on marine facilities, causing drift or even overturning; blocking the mesh holes of aquaculture cages, cages, seines, etc., affecting the exchange of water in the internal and external environment, reducing the internal environment. Dissolved oxygen hinders the normal growth and development of breeding objects.

[0003] Ulva lactuca is edible and medicinal. It mostly grows on rocks or bogs in middle and low tidal zones, and can also grow on...

Examples

Embodiment 1

[0023] The culture medium is natural seawater, and after the seawater is filtered, boiled and sterilized, and cooled to room temperature, it is placed in a high-temperature sterilized vessel for use.

[0024] The collected fresh Ulva was placed in sterilized seawater for temporary maintenance.

[0025] Take about 2 cm of yellow-green algae with brown edges on a glass slide, add a small amount of sterilized seawater dropwise, and place it on a Nikon MD-TS100 microscope stage.

[0026] The spore collection test was divided into three groups: group A, light intensity 3500Lx; group B, light intensity 500-3000Lx; group C, control group, indoor natural light irradiation. The light time is 10 minutes, and seawater is added dropwise in time to prevent evaporation from drying up the algae. Microscopic examination, use a pipette to absorb the released spores, that is, to absorb them with a pipette, and place them in a 50ml small beaker for later use. Repeat the above steps to continue...

Embodiment 2

[0029] The culture medium is natural seawater, and after the seawater is filtered, boiled and sterilized, and cooled to room temperature, it is placed in a high-temperature sterilized vessel for use.

[0030] The collected fresh Ulva was placed in sterilized seawater for temporary maintenance.

[0031] Take about 2 cm of yellow-green algae with brown edges on a glass slide, add a small amount of sterilized seawater dropwise, place it on a Nikon MD-TS100 microscope stage, and stimulate it for 5 minutes with a light intensity of 4000 Lx. Microscopic examination found that the zoospores were sucked up with a straw and placed in a 50ml small beaker for later use. Repeat the above steps to continue collecting spores 2 times. Replace with fresh algae fragments, and repeat the above steps to collect spores.

[0032] Take a small amount of spore liquid in a beaker and count it on a hemocytometer to determine the number of zoospores per milliliter of water, and dilute it with sterili...

Embodiment 3

[0037] The culture medium is natural seawater, and after the seawater is filtered, boiled and sterilized, and cooled to room temperature, it is placed in a high-temperature sterilized vessel for use.

[0038] The collected fresh Ulva was placed in sterilized seawater for temporary maintenance.

[0039] Take about 2 cm of yellow-green algae with brown edges on a glass slide, add a small amount of sterilized seawater dropwise, place it on a Nikon MD-TS100 microscope stage, and stimulate it for 3 minutes with a light intensity of 420Q Lx. Microscopic examination found that the release of zoospores was sucked up with a straw, and placed in a 50ml small beaker for later use. Repeat the above steps to continue collecting spores 2 times. Replace with fresh algae fragments, and repeat the above steps to collect spores.

[0040] Take a small amount of spore liquid in a beaker and count it on a hemocytometer to determine the number of zoospores per milliliter of water, and dilute it wit...