Method for detecting melamine based on oligonucleotide-non-labeled nano-Au
An oligonucleotide and melamine technology, which is applied in the interdisciplinary field, can solve the problems of time-consuming, labor-intensive, and no commercialization of reagents, and achieves the effects of good selectivity, high sensitivity and low cost.
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[0023] Taking the detection of melamine in raw milk as an example to illustrate the detection method, the steps are as follows:
[0024] 1) All the instruments used for the synthesis of gold nanoparticles need to use freshly prepared aqua regia (1:3, V 浓硝酸 / V 浓盐酸 ) soaked for 24 hours, washed with ultrapure water for later use.
[0025] 2) Heat 100mL ultrapure water to boil, add 1.88mL chloroauric acid (2%, w / w), after boiling, add 10.0mL trisodium citrate (the mass of trisodium citrate is 0.1141g). Stop heating after 10.0 minutes, continue to stir for 15.0 minutes, and naturally cool to room temperature (20-25° C.). The molar ratio of described chloroauric acid to trisodium citrate is 1: 3.88. The prepared nano-gold sol has a particle size of 13nm and a concentration of 4.86×10 -9 M.
[0026] 3) Dissolving the specific sequence oligonucleotide in the buffer solution to obtain the reaction solution B. The specific sequence oligonucleotide is a single-stranded oligonucle...
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