Method for detecting melamine based on oligonucleotide-non-labeled nano-Au

An oligonucleotide and melamine technology, which is applied in the interdisciplinary field, can solve the problems of time-consuming, labor-intensive, and no commercialization of reagents, and achieves the effects of good selectivity, high sensitivity and low cost.

Active Publication Date: 2012-03-21
常熟紫金知识产权服务有限公司
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  • Abstract
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  • Application Information

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Problems solved by technology

Although a similar detection method (Kelong Ai, Yanlan Liu, and Lehui Lu, Journal of the American Chemical Society 2009, 131(27), 9496-9497.) was reported using this principle not long ago, the method they reported not only requires Gold nanoparticles are labeled with sulfhydryl reagents, and the reagents are not commercialized and need to be synthesized by themselves, which is not only labor-intensive, time-consuming and labor-intensive, but also its purity directly affects detection sensitivity and selectivity

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  • Method for detecting melamine based on oligonucleotide-non-labeled nano-Au
  • Method for detecting melamine based on oligonucleotide-non-labeled nano-Au
  • Method for detecting melamine based on oligonucleotide-non-labeled nano-Au

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Embodiment 1

[0023] Taking the detection of melamine in raw milk as an example to illustrate the detection method, the steps are as follows:

[0024] 1) All the instruments used for the synthesis of gold nanoparticles need to use freshly prepared aqua regia (1:3, V 浓硝酸 / V 浓盐酸 ) soaked for 24 hours, washed with ultrapure water for later use.

[0025] 2) Heat 100mL ultrapure water to boil, add 1.88mL chloroauric acid (2%, w / w), after boiling, add 10.0mL trisodium citrate (the mass of trisodium citrate is 0.1141g). Stop heating after 10.0 minutes, continue to stir for 15.0 minutes, and naturally cool to room temperature (20-25° C.). The molar ratio of described chloroauric acid to trisodium citrate is 1: 3.88. The prepared nano-gold sol has a particle size of 13nm and a concentration of 4.86×10 -9 M.

[0026] 3) Dissolving the specific sequence oligonucleotide in the buffer solution to obtain the reaction solution B. The specific sequence oligonucleotide is a single-stranded oligonucle...

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Abstract

The invention discloses a method for detecting melamine based on oligonucleotide-non-labeled nano-Au, comprising the following steps of: 1) preparing nano-Au colloidal sol with certain grain diameter, namely, reaction liquid A; 2) dissolving oligonucleotide solution with special sequence into buffer solution, and obtaining reaction liquid B; 3) adding solution to be detected into the reaction liquid B for reaction; and 4) adding the reaction liquid A into the reaction liquid in the step 3), and detecting the melamine by colorimetry. The detection method is simple, convenient and rapid (the detection process is not longer than 10min), high in sensitivity, good in selectivity and low in cost, can reach the detection requirement in provisional management limited quantity regulation of melamine in milk and dairy products of China without needing large-scale instruments and equipment, and can realize field rapid detection for raw materials and products.

Description

technical field [0001] The invention relates to the interdisciplinary fields of biology, medicine, food science, chemistry and other subjects. More specifically, it relates to a method for detecting melamine based on oligonucleotide-non-labeled nano gold. Background technique [0002] The harm of melamine to humans and animals has attracted widespread attention. The fake protein incidents of liquid milk and milk powder are mainly due to the fact that some unscrupulous people add melamine to milk or milk powder to increase the nitrogen content of milk products. Fake protein. However, after melamine enters the human body, a substitution reaction (hydrolysis) occurs to generate cyanuric acid. Cyanuric acid and melamine form a large network structure, and form insoluble salts with urine to cause stones, which pose a serious threat to health. On October 7, 2008, the Ministry of Health, the Ministry of Industry and Information Technology, the Ministry of Agriculture, the State A...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/68G01N21/31
Inventor 何治柯黄晖李丽
Owner 常熟紫金知识产权服务有限公司
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