A kind of immunochromatography test paper assembled with chitin or chitosan fiber membrane as colloidal marker pad and preparation method thereof

An immunochromatography, chitosan fiber technology, applied in the field of biological detection and biomaterials, can solve the problem of no special emphasis, and achieve the effect of improving stability, improving test stability and sensitivity, and excellent detection performance

Active Publication Date: 2019-12-06
RES INST OF ZHEJIANG UNIV TAIZHOU
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the currently disclosed prior art, the commonly used materials for this component are glass fiber membranes, filter paper or polypropylene fiber non-woven fabrics. Although they can meet the general needs of use, there is no special emphasis on the excellent properties of the above materials.

Method used

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  • A kind of immunochromatography test paper assembled with chitin or chitosan fiber membrane as colloidal marker pad and preparation method thereof
  • A kind of immunochromatography test paper assembled with chitin or chitosan fiber membrane as colloidal marker pad and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Embodiment 1 adopts the pregnancy test paper of chitin or chitosan fiber film colloidal gold pad

[0030] 1. Select chitin / chitosan raw materials with 30% deacylation degree;

[0031] 2. Using mechanical disc grinding method, water, 5% sodium hydroxide by mass fraction, and 5% sodium carbonate by mass fraction are used as auxiliary materials to prepare the chitin / chitosan mixture into wet slurry; After the slurry is taken from the slurry tank, the film material with a thickness of 2 mm is obtained through the electric heating drum, the film winding drum and the film receiving drum, and is washed and dried for later use;

[0032] 3. Prepare colloidal gold-labeled human chorionic gonadotropin (HCG) mouse antibody 1 solution.

[0033] 4. The solution is evenly sprayed on the chitin / chitosan membrane material at a ratio of 1 milliliter to 15 square centimeters, and dried for 2 hours in an environment with a humidity of 25% and a temperature of 30 degrees Celsius to make a ...

Embodiment 2

[0036] Embodiment 2 adopts the methamphetamine (ice poison) competitive inhibition method detection test paper of chitin or chitosan fiber film colloidal gold pad

[0037] 1. Select pure chitosan raw materials;

[0038] 2. The chitosan raw material is dissolved in acetic acid, and the chitosan fiber is obtained through a wet spinning process, and a dry film material with a thickness of 1.5 mm is obtained through a general non-woven fabric weaving process for future use.

[0039] 3. Prepare methamphetamine monoclonal antibody-colloidal gold solution.

[0040] 4. After soaking the chitosan film material in the antibody-colloidal gold solution, take it out, and dry it for 6 hours in an environment with a humidity of 35% and a temperature of 20 degrees Celsius to make a colloid release pad.

[0041] 5. In an environment with a humidity of 35% and a temperature of 20 degrees Celsius, the nitrocellulose coated with T-line (coated with methamphetamine-bovine serum albumin) and C-lin...

Embodiment 3

[0043] Embodiment 3 adopts the soybean transgenic Bt-Cry1Ab / Ac detection test paper of chitin or chitosan fiber membrane colloidal carbon pad

[0044] 1. Select chitin / chitosan raw materials with 5% deacylation degree;

[0045] 2. Chitin / chitosan raw materials were dissolved in ionic solvent LiCl / DMAc to make a solution, and the solution was cast on a polytetrafluoroethylene template. After the solvent was volatilized, it was heated and dried to obtain a chitin / shell with a thickness of 2.5 mm. The polysaccharide in-situ membrane material is ready for use;

[0046] 3. Prepare colloidal carbon-labeled Bt-Cry1Ab / Ac monoclonal antibody solution.

[0047] 4. The solution is evenly sprayed on the chitin / chitosan membrane material in a ratio of 1 milliliter to 16 square centimeters, and dried for 5 hours in an environment with a humidity of 20% and a temperature of 27 degrees Celsius to make a colloid release pad.

[0048] 5. Cut a chitin / chitosan membrane of appropriate size as a...

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Abstract

Provide a kind of immunochromatography test paper and its preparation method using chitin or chitosan fiber membrane as a colloidal marker pad assembly preparation, the test paper has the characteristics of uniform adsorption of negatively charged colloidal particles, hydrophilic and stable flow, and neutrality of biological macromolecules. It has the advantages of high test stability and sensitivity, and long validity period. The colloid marking pad of the present invention is mixed with pure chitin fiber and deacylated chitosan fiber in any proportion, and is prepared into a fiber film with a thickness of 0.2-4mm by pulping method, spinning method or solution method, which can load more A colloidal particle marker, and a test paper prepared by assembling with different detection membranes, can be widely used in physiological health, disease detection, drug, doping detection, transgenic protein detection and other fields.

Description

technical field [0001] The invention belongs to the fields of biological detection and biomaterials, in particular to an immunochromatographic test paper using chitin or chitosan fiber membrane as a colloid carrier. Background technique [0002] Immunochromatography (immunochromatography) is a rapid diagnostic technique that has emerged and is widely used in recent years. Its principle is to fix the specific antibody on a certain zone of the nitrocellulose membrane first, and when the dry nitrocellulose end is immersed in the sample ( That is, the solution that may contain the target detection substance), the sample will move forward along the membrane due to capillary action, and when it moves to the area where the antibody is immobilized, the corresponding antigen in the sample will specifically bind to the antibody. Immunocolloidal gold or immunoenzyme-linked staining can make this area show a certain color, so as to realize specific immunodiagnosis. Immunochromatography...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/531G01N33/558
CPCG01N33/531G01N33/558
Inventor 徐沁黄然
Owner RES INST OF ZHEJIANG UNIV TAIZHOU
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