Luminol-gallium nano assembly as well as preparation method and application thereof

A nano-assembly and assembly technology, which is applied in the field of chemical biosensing and biological detection, can solve the problems of limited practical application and high cost of synthesizing nanomaterials, and achieve the effects of easy operation, low detection limit and good selectivity

Active Publication Date: 2020-08-25
CHONGQING NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the tedious process and high cost of synthesizing nanomaterials greatly limit their practical applications

Method used

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  • Luminol-gallium nano assembly as well as preparation method and application thereof
  • Luminol-gallium nano assembly as well as preparation method and application thereof
  • Luminol-gallium nano assembly as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034]A luminol-gallium nanoassembly was prepared by a one-pot method, and the specific preparation method was as follows:

[0035] Add 100 μL of gallium trichloride solution (1 mM) and 40 μL of luminol solution (20 mM) into a 1.5 mL plastic vial, mix them thoroughly, and obtain a luminol-gallium nanoassembly after more than 1 min at room temperature.

[0036] The TEM and SEM images of the luminol-gallium nanoassembly prepared by the above method are as follows: figure 1 Shown in A and B. From figure 1 The results indicated that the luminol-gallium nanoassemblies were formed by small particles, and the small particles self-assembled together and aggregated to form chain or network nanoassemblies. The prepared luminol-gallium nanoassembly can be stored stably for more than one month in a refrigerator at 4°C, indicating that the luminol-gallium nanoassembly has good stability.

[0037] In addition, because the above-mentioned reaction mainly comes from the formation of self-a...

Embodiment 2

[0039] 3,3,5,5-Tetramethylbenzidine (TMB) was used as a peroxidase substrate to test the peroxide mimetic enzyme activity of luminol-gallium nanoassemblies. The specific method was as follows:

[0040] The luminol-gallium nanoassembly prepared in the example was added to TMB-H with 3,3,5,5-tetramethylbenzidine (TMB) as a peroxidase substrate 2 o 2 Formation of luminol-gallium nanoassembly-TMB-H in the system (the system is HAc-NaAc with a concentration of 0.04M at pH=4.0) 2 o 2 system, and then to the luminol-gallium nanoassembly-TMB-H 2 o 2 Add pyrophosphate (PPi) to the system, test the system respectively (the concentration of luminol in the system is 0.8mM, Ga 3+ The concentration is 0.1mM, H 2 o 2 The concentration of TMB is 0.2M, the concentration of TMB is 1.0mM) UV-visible absorption before and after adding luminol-gallium nanoassembly, the test results are as follows figure 2 Shown (where a is TMB-H 2 o 2 system, b is luminol-gallium nanoassembly-TMB-H 2 o ...

Embodiment 3

[0042] In the colorimetric method, PPi is detected by luminol-gallium nanoassembly, and the influence results of the conditions in the test are as follows:

[0043] To a concentration of 0.8mM luminol, a concentration of 0.2M H 2 o 2 and a concentration of 1 mM H 2 o 2 Ga 3+ , gradually increasing Ga 3+ The content of Ga in the determination system 3+ Absorbance in the system when the concentration is 0.01mM, 0.02mM, 0.05mM, 0.1mM, 0.6mM, 0.8mM, the percentage of absorbance reduction ((A 0 -A) / A 0 ) with Ga 3+ The concentration change trend graph is as follows image 3 Shown (A 0 For Luminol-Gallium Nanoassembly-TMB-H 2 o 2 The absorbance of the system, A is the luminol-gallium nanoassembly-TMB-H 2 o 2 Absorbance after adding PPi to the system). Depend on image 3 It can be seen that the percentage of absorbance decrease ((A 0 -A) / A 0 ) with Ga 3+ The trend of concentration change is to increase first and then decrease. 3+ The absorbance drops to the minimum...

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Abstract

The invention relates to a luminol-gallium nano assembly as well as a preparation method and application thereof, and belongs to the technical field of chemical and biological sensing and biological detection. According to the invention, a luminol-gallium nano assembly which is formed by self-assembly of luminol and Ga < 3 + > and is of a chain-shaped or net-shaped structure is prepared by adopting a one-pot method; typical 3, 3, 5, 5-tetramethyl benzidine (TMB) as peroxidase is taken as a substrate, activity similar to peroxide mimic enzyme is shown, and the luminol-gallium nano assembly triggers colorless TMB to be oxidized into blue oxidized TMB in the presence of H2O2; besides, due to coordination between PPi and Ga < 3 + >, oxidation of TMB catalyzed by the luminol-gallium nano-assembly can be inhibited, blue formed by blue oxidation of TMB becomes light or even disappears, so that the luminol-gallium nano-assembly provided by the invention realizes colorimetric detection of PPi and has the characteristics of low detection limit and good selectivity.

Description

technical field [0001] The invention belongs to the technical field of chemical biosensing and biological detection, and in particular relates to a luminol-gallium nano assembly and a preparation method and application thereof. Background technique [0002] Natural enzymes are effective catalysts for thousands of biological reactions, but their poor stability in non-physiological environments, high production costs, and difficult storage have never limited their practical applications. Similar to natural enzymes, nanomaterials are considered as artificial enzymes with satisfactory efficiency, stability, and selectivity. Therefore, the application of nanomaterials as enzymes is a growing field of research worldwide. To date, various nanomaterials have been used as enzymes, including quantum dots, transition metal dichalcogenides, noble metal nanoparticles / nanoclusters, composite materials, etc. In the existing report, Liu et al. synthesized photosensitive metal-organic fram...

Claims

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Application Information

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IPC IPC(8): G01N21/78G01N21/31B82Y40/00
CPCG01N21/78G01N21/31B82Y40/00
Inventor 祁文静田雪吴狄
Owner CHONGQING NORMAL UNIVERSITY
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