Lentiviral gene transfer vector, preparation method and application thereof

A technology of gene transfer and lentivirus, which is applied in the field of genetic engineering, can solve problems affecting research and product development in the field, and there is no standardized and unified technology, so as to achieve the effect of stable protein expression, increased transfection efficiency, and increased expression
CN101705246AInactive Publication Date: 2010-05-12HARBIN VETERINARY RES INST CHINESE ACADEMY OF AGRI SCI
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
HARBIN VETERINARY RES INST CHINESE ACADEMY OF AGRI SCI
Publication Date
2010-05-12
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention discloses an equine infectious anemia virus (EIAV) gene transfer vector, a constructed method and an application thereof. The vector of the invention comprises a CMV / R / U5 promoter, a 5' non-translated region homing sequence, a partial 5' gag gene coding region sequence, a central polypurine sequence, an Rev reaction element of EIAV, a CMVIE / chicken beta-actin promoter, a reporter gene, WPRE of WHBV, a polyclone locus, a poly (A) signal before EIAV 3'LTR, complete 3'LTR and partial sequence of eukaryon expression vector. The vector of the invention possesses common advantages of lentiviral vector, lacks auxiliary genes of all the wild-type virus, can carry one or a plurality of selected genes to transmit to target cells, can insert with target DNA sequence with large fragments and can lead the foreign genes and reporter genes to express at a high level in the cells.
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Description

technical field

[0001] The present invention relates to a lentiviral gene transfer vector, in particular to a defective lentiviral gene transfer vector derived from Equine Infectious Anemia Virus (EIAV) and its construction method. The application in gene transfer of cells in vitro belongs to the field of genetic engineering. Background technique

[0002] The currently constructed EIAV gene transfer vectors are mainly derived from American strains reported abroad, and there are large differences between American strains and Chinese strains, with nucleotide homology between 78.4% and 80%. There is no related report of the same type in China, and no one has used woodchuck hepatitis virus post-transcriptional regulatory element (WPRE) and chicken β-actin promoter (CAGp) to enhance the expression of the reporter gene on the EIAV gene transfer vector. At present, there is no commercialized EIAV gene transfer vector in China, and there is no standardized and unified technology, w...

Claims

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