Novel method for splitting 2,4,5-dichlorprop enantiomers by capillary electrophoresis apparatus
A technology of dropping propionic acid and enantiomers, applied in the field of chiral herbicides 2, can solve the problems of soil compaction, increase of drug-resistant weed populations, affecting the sustainable development of agriculture and human health and safety, and achieves a simple separation method. Effect
Active Publication Date: 2013-04-17
ZHEJIANG UNIV OF TECH
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Problems solved by technology
According to statistics, the annual herbicide use area in my country is 2 million hm 2 Increasingly, the use of such a large amount of chemical herbicides, on the one hand, it has made a very important contribution to our agricultural development, but at the same time it has also caused a series of environmental problems, such as the increase of drug-resistant weed populations, soil compaction, water pollution etc., seriously affecting the sustainable development of agriculture and human health and safety
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Embodiment 1
[0020] (1) Sample preparation: 5 mg of 2,4,5-propionic acid was weighed and dissolved in 1 ml of methanol to prepare a 5 mg / ml mother solution. Then take 10 μl and add it to 990 μl distilled water, and configure 1 ml into a 0.05 mg / ml sample solution.
[0021] (2) Preparation of running buffer solution: 2.884g of sodium dodecyl sulfate (SDS) was weighed and dissolved in 50ml of water to prepare a solution with a concentration of 200mmol / L. Take 0.685ml of H 3 PO 4 Dilute to 50ml with distilled water to make a solution with a concentration of about 200mmol / L. Then take 12.5ml of the above solution and dilute to 50ml, and make H 3 PO 4 Concentration and SDS concentration are both 50 mM buffer solution, and the pH is adjusted to about 2 with saturated NaOH solution. Finally, take 5ml of the previously prepared buffer solution with a pH of 2, weigh 195mg of γ-CD and dissolve it in it, and prepare the buffer solution with added chiral selector for the final operation. The run...
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Abstract
The invention discloses a novel method for splitting 2,4,5-dichlorprop enantiomer by utilizing a capillary electrophoresis apparatus, comprising the following steps of: (1) mixing racemic 2,4,5-dichlorprop, an organic modifying agent and an aqueous solution to prepare a sample solution, wherein the organic modifying agent is methanol or acetonitrile; (2) preparing a lauryl sodium sulfate-phosphate buffer solution, adjusting the pH value to be within the range of 2 to 3, then adding a chiral selecting agent gamma-cyclodextrin and controlling the quality final concentration of the gamma-cyclodextrin to be 3 to 5 percent; and (3) putting the sample solution and the lauryl sodium sulfate-phosphate buffer solution containing the gamma-cyclodextrin into the capillary electrophoresis apparatus, and applying a reverse voltage for splitting. The splitting method of the 2,4,5-dichlorprop enantiomer is simple and accurate and provides a technical support for accurately and truly knowing and evaluating the selective difference of the 2,4,5-dichlorprop enantiomer.
Description
(1) Technical field [0001] The invention relates to an enantiomer resolution method of a chiral herbicide 2,4,5-dipropionic acid, which belongs to the technical field of environmental pollutant analysis. (2) Background technology [0002] Herbicides, also known as herbicides, are a class of chemicals used to kill plants. These agents can selectively act on specific targets, so that other crops useful to humans are not harmed, or suffer less damage. According to statistics, the annual herbicide use area in my country is 2 million hm 2 Increasingly, the use of such a large amount of chemical herbicides, on the one hand, it has made a very important contribution to our agricultural development, but at the same time it has also caused a series of environmental problems, such as the increase of drug-resistant weed populations, soil compaction, water pollution etc., have seriously affected the sustainable development of agriculture and human health and safety. [0003] 2,4,5-Di...
Claims
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IPC IPC(8): G01N27/447
Inventor 张全赵美蓉刘维屏王萃
Owner ZHEJIANG UNIV OF TECH