New method for separating 2,4-dichlorprop enantiomer by capillary electrophoresis
A technology of dipropionic acid and enantiomers, applied in the field of chiral herbicides, can solve problems such as soil compaction, impact on sustainable agricultural development, human health and safety, and increase in the population of drug-resistant weeds, and achieve a simple separation method Effect
Active Publication Date: 2013-07-24
ZHEJIANG UNIV OF TECH
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Problems solved by technology
According to statistics, the annual herbicide use area in my country is 2 million hm 2 Increasingly, the use of such a large amount of chemical herbicides, on the one hand, it has made a very important contribution to our agricultural development, but at the same time it has also caused a series of environmental problems, such as the increase of drug-resistant weed populations, soil compaction, water pollution etc., seriously affecting the sustainable development of agriculture and human health and safety
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Embodiment 1
[0020] (1) Sample preparation: Weigh 5 mg of 2,4-dipropionic acid and dissolve it in 1 ml of methanol to prepare a 5 mg / ml mother solution. Then take 10 μl of the mother solution and add it to 990 μl of distilled water, and configure 1 ml into a 0.05 mg / ml sample solution.
[0021] (2) Preparation of running buffer solution: 2.884g of sodium dodecyl sulfate (SDS) was weighed and dissolved in 50ml of water to prepare a solution with a concentration of 200mmol / L. Take 0.685ml of H 3 PO 4 Add it to 50ml of water to make a solution with a concentration of about 200mmol / L. Then take 12.5ml of the above solution and dilute to 50ml, and make H 3 PO 4 Concentration and SDS concentration are both 50 mM buffer solution, and the pH is adjusted to about 2 with saturated NaOH solution. Finally, take 5ml of the previously prepared buffer solution with a pH of 2, weigh 130mg of γ-CD and dissolve it in it, and prepare the buffer solution with added chiral selector for the final operation...
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Abstract
The invention discloses a new method for separating 2,4-dichlorprop enantiomer by capillary electrophoresis, comprising the following steps: (1) mixing racemic 2,4-dichlorprop, organic modifier and hydrosolvent to prepare a sample solution, wherein the organic modifier is methanol or acetonitrile; (2) preparing a sodium dodecyl sulfate-phosphoric acid buffer solution, adjusting pH to 2-3, then adding a chiral selector gamma-cyclodextrin, and maintaining a final mass concentration of the gamma-cyclodextrin at 2-4%; and (3) adding the sample solution and the gamma-cyclodextrin containing sodiumdodecyl sulfate-phosphoric acid buffer solution into a capillary electrophoresis apparatus, and applying a reverse voltage for separation. The 2,4-dichlorprop enantiomer separation method of the invention is simple and accurate, and provides a technical support for real understanding and evaluation of the substances on enantiomer selectivity difference.
Description
(1) Technical field [0001] The invention relates to an enantiomer resolution method of a chiral herbicide 2,4-dipropionic acid, which belongs to the technical field of environmental pollutant analysis. (2) Background technology [0002] Herbicides, also known as herbicides, are a class of chemicals used to kill plants. These agents can selectively act on specific targets, so that other crops useful to humans are not harmed, or suffer less damage. According to statistics, the annual herbicide use area in my country is 2 million hm 2 Increasingly, the use of such a large amount of chemical herbicides, on the one hand, it has made a very important contribution to our agricultural development, but at the same time it has also caused a series of environmental problems, such as the increase of drug-resistant weed populations, soil compaction, water pollution etc., have seriously affected the sustainable development of agriculture and human health and safety. [0003] 2,4-Dichlo...
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IPC IPC(8): G01N27/447
Inventor 赵美蓉张全刘维屏王萃
Owner ZHEJIANG UNIV OF TECH