Method for rapid propagation of rauwolfia yunnanensis by tissue culture technology

A tissue culture and Rauwolfia technology, applied in the field of plant tissue culture, can solve the problems of difficult rooting of cuttings, low reproduction and germination rate, etc., and achieve the effects of large-scale planting, convenient collection and good genetic stability.

Inactive Publication Date: 2010-06-16
CENTRAL SOUTH UNIVERSITY OF FORESTRY AND TECHNOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

It solves the problem of low reproductive germination rate of Rauvolwolfia yunnanensis a

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] 1) Extraction and disinfection of explants: select young shoots about one month old as explants, cut off the leaves (keep the petioles), wash them with detergent for 20 minutes, then rinse them with tap water for 2 hours, and put them on the ultra-clean workbench. Disinfect with 70% alcohol for 8 seconds, 0.1% mercuric chloride for 10 minutes, rinse with sterile water for 5-6 times, and cut into 1cm-long stems with buds.

[0020] 2) Induction culture Inoculate the sterilized bud-bearing stem section on the starting medium of MS+6-BA 2.0 mg / L+NAA 0.05 mg / L on the ultra-clean workbench to induce the production of axillary buds, and the culture condition is 25°C , photoperiod 16h / 8h, light intensity 2000lx, 4-6cm high starter seedlings were obtained after 4 weeks.

[0021] 3) Proliferation culture: use the terminal buds and budded stem sections of the starting seedlings as explants, inoculate them on the proliferation medium of MS+6-BA 2.5 mg / L+NAA 0.1 mg / L, and the cultur...

Embodiment 2

[0025] The specific implementation process is the same as example 1. The induction medium formula is MS+6-BA 3.0 mg / L+NAA0.1mg / L; the proliferation medium formula is the same as the induction medium, which is MS+6-BA 3.0+NAA 0.3mg / L; the proliferation coefficient is 8-10 , the rooting medium formula is 1 / 2 MS+IBA 1.0mg / L; the rooting rate is above 90%; the transplanting survival rate is 90%.

Embodiment 3

[0027] The specific implementation process is the same as example 1. Among them, the induction medium formula is MS+6-BA4.0+NAA0.1mg / L; the proliferation medium formula is MS+6-BA 5.0+NAA 0.5mg / L; the proliferation coefficient is 10-12; the rooting medium formula is 1 / 2 MS+NAA0.5+IBA 0.5mg / L; rooting rate 100%; transplanting survival rate 90%.

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Abstract

The invention discloses a method for rapid propagation of rauwolfia yunnanensis by tissue culture technology, belonging to the technical field of plant tissue culture. The method is divided into four stages: induction, propagation, root creation and seedling hardening-off. Devilpepper youngshoot with stolon section serves as an explant; after being disinfected, the explant is inoculate to an initiation culture medium to induce auxiliary buds to come out, the composition of the initiation culture medium is MS+2.0-4.0mg/LBA+0.05-0.1mg/LNAA.Enrichment culture is MS+2.5-5.0mg/LBA+0.1-0.5mg/LNAA, multiplication coefficient is 8-12 and passage is repeated every 4-5 weeks. A rooting medium is a 1/2MS culture medium in which 1.0mg/LNAA or IBA is added or NAA+IBA=1.0mg/L, after 5-6 weeks, root seedlings are transplanted to a seedling hardening-off ground substance, moisture preservation, overshadowing and seedling hardening-off are carried out for 3-4 weeks at 20-30 DEG C on the root seedlings, then the root seedlings are transplanted or planted in the field with a survival rate of 90%.The method of the invention features simple operation, rapid propagation, good genetic stability, thus providing technological supports for industrialized nursery process.

Description

technical field [0001] The invention belongs to the field of plant tissue culture, and in particular relates to a method for rapidly propagating Rauwolfia yunnanensis by tissue culture technology. Background technique [0002] Rauwolfia yunnanensis is an important medicinal plant. Its root contains dozens of alkaloids such as reserpine, yohimbine, and snakeroot, which are raw materials for treating hypertension; Mined as a source of medicine, wild Rauvolwolfia yunnanensis has been quite rare. In order to meet the domestic and international demand for raw materials of Rauwolfia and protect the only wild Rauwolfia resources, artificial planting of Rauwolfia has a good development prospect. The seed reproduction and germination rate of Rauvolwolfia yunnanensis is low, and the rooting of cuttings is difficult, which has become a bottleneck for large-scale planting. At present, there are no reports from other research institutions on the tissue culture of Rauwolfia yunnanensis ...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 龙绛雪曹福祥黄铁坚徐庆军
Owner CENTRAL SOUTH UNIVERSITY OF FORESTRY AND TECHNOLOGY
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