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Chlorella extractive composition and preparation method thereof

A composition and extract technology, applied in the field of γ-aminobutyric acid, can solve problems such as allergic reactions

Inactive Publication Date: 2010-06-23
TAIWAN CHLORELLA MFG
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Most of the ACE inhibitors used in medicine as antihypertensive drugs are short-chain peptide analogs, such as captopril, enalapril, alacepril and lisinopril ( lisinopril), but there will be dry cough, dizziness, allergic reactions and other side effects after taking

Method used

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  • Chlorella extractive composition and preparation method thereof
  • Chlorella extractive composition and preparation method thereof
  • Chlorella extractive composition and preparation method thereof

Examples

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Embodiment 1

[0038] The cultivation and freeze-drying preparation of embodiment one lactic acid bacteria

[0039] Take 100 microliters of the bacterial solution from -80°C, add it to 10ml of MRS, and incubate at 37°C for 6-12 hours. After measuring the OD value of 0.8-1.0, take 1ml and add it to a conical flask containing 1 liter of MRS. In this method, cultivate again at 37°C for 6-12hrs, and when the OD value is 0.8-1.0, centrifuge at 7000×g for 10 minutes at 4°C, discard the upper MRS after centrifugation, and add sterilized 0.85% NaCl (3-5 After shaking evenly, centrifuge twice at 7000×g, 10min, 4°C, pour off the NaCl, add skimmed fresh milk as the base, and spread and freeze-dry.

Embodiment 2

[0040] The preparation of embodiment two Chlorella extract

[0041] Take an appropriate amount (such as 30-70 grams) of Cyclocystic Chlorella powder, add 15 grams of sucrose and add 450 milliliters of deionized water, heat in a boiling water bath for 30 minutes for sterilization, and add an appropriate amount (such as 0.5-5% by weight) of Mix lactic acid bacteria and commercial protease in an incubator at 42°C for 0 to 40 hours, then heat in boiling water for 30 minutes, after cooling, centrifuge at 6,000×g for 10 minutes, filter the supernatant with No. 2 filter paper, and finally freeze-dry Machine freeze-dried to make powder.

Embodiment 3

[0042] Soluble protein content determination (Peterson, 1979) of embodiment three Chlorella extracts

[0043] Take 0.3 g of Chlorella clastans fermentation hydrolyzate freeze-dried powder, quantify it to 10 ml with deionized water, filter it through a 0.2 micron filter membrane and then dilute appropriately, take 0.1 ml, 0.5 ml of A reagent and 4 ml of B reagent (Bio- Rad DC Protein Assay Kit, Bio-Rad Laboratories Inc., USA) were mixed, allowed to stand for 15 minutes, then measured its absorbance value at a wavelength of 750 nanometers with a spectrophotometer, obtained by fetal bovine serum albumin (Bovine Serum albumin) standard substance The obtained standard calibration line is converted to the content of soluble protein, and the unit is mg / g.

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Abstract

The invention discloses a chlorella extractive and a preparation method thereof. The chlorella extractive includes an active peptide and an inhibitory nerve transmitter, can effectively inhibit the activity of an angiotension I-converting enzyme and can be further used for developing a composition for adjusting the physiological effect of blood pressure.

Description

technical field [0001] The invention uses green algae as raw material, and uses protease to assist lactic acid bacteria to ferment the green algae to produce blood pressure-lowering peptide (peptide) and neurotransmitter gamma-aminobutyric acid (GABA). At the same time, the green algae fermented hydrolyzate was hydrolyzed by simulating gastrointestinal digestive enzymes to further determine the degradation of peptide activity and its inhibitory ability to vasopressin-converting enzyme. Then, the active peptide was purified by colloid chromatography and high performance liquid chromatography (HPLC), and its peptide sequence was identified. Background technique [0002] Metabolic syndrome (metabolic syndrome) mainly includes hypertension or hypertension with high blood pressure but not reaching the diagnostic criteria of hypertension; dyslipidemia including high blood triglycerides and low high-density lipoprotein cholesterol (dyslipidemia); diabetes Or high fasting blood sug...

Claims

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Application Information

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IPC IPC(8): A61K36/05A61K38/01A61K38/05A61P9/12C12P21/00C12P39/00C12R1/46C12R1/245C12R1/23C12R1/225C12R1/00
Inventor 蔡震寿萧秀玟叶锦熙
Owner TAIWAN CHLORELLA MFG
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