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Method for measuring trace Hg2+ by using aptamer modified nano gold rhenium catalysis-tellurium particle resonance scattering spectrum

A technique of resonance scattering and spectrometry, applied in fluorescence/phosphorescence, analysis through chemical reaction of materials, material excitation analysis, etc., can solve the problems of difficult operation, cumbersome steps, low sensitivity, etc., and achieve simple operation and excellent reaction conditions Easy and selective effect

Inactive Publication Date: 2010-07-14
GUANGXI NORMAL UNIV
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Problems solved by technology

Some of the above methods have low sensitivity, and some have cumbersome steps and are not easy to operate.

Method used

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  • Method for measuring trace Hg2+ by using aptamer modified nano gold rhenium catalysis-tellurium particle resonance scattering spectrum
  • Method for measuring trace Hg2+ by using aptamer modified nano gold rhenium catalysis-tellurium particle resonance scattering spectrum

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Embodiment Construction

[0022] (1) prepare gold-rhenium composite nanoparticles according to the method described in the preceding step (1);

[0023] (2) prepare the AuRessDNA probe according to the method described in the preceding step (2);

[0024] (3) prepare the filtrate according to the aforementioned steps (3);

[0025] (4) Preparation of known Hg 2+ Concentration catalytic reaction measurement system: In a 5mL stoppered graduated test tube, add 0.3mL0.012mol / L Na 2 TeO 4 Solution, 0.15mL 0.3mol / L tartaric acid solution; 30μL containing different concentrations of Hg 2+ The filtrate, 0.7mL0.9mol / L SnCl 2 Solution, mix well, and dilute to 3mL. Place in a constant temperature water bath and heat at 65 °C for 10 min. Rapid cooling to room temperature in running water. Use a fluorescence spectrophotometer volt=400v, incident slit width=exit slit width=5nm, λ ex -λ em = Under the condition of Δλ=0, synchronously scan to obtain the resonance scattering spectrum, and measure the resonance sc...

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Abstract

The invention discloses a method for detecting trace Hg2+ by using aptamer modified nano gold rhenium catalysis-tellurium particle resonance scattering spectrum. T-T mismatch in an ssDNA sequence can be stabilized by using Hg2+ so that the ssDNA cannot stabilize AuRe composite nano particles and the AuRe composite nano particles are gathered under the high-concentration electrolyte condition, and the phenomenon and a nano gold rhenium catalysis reaction are organically combined; and the tellurium particles generated by the catalytic reaction have resonance scattering; and the Hg2+ and the resonance scattering strength are in linear relation. A test system with known mercury concentration and a reagent vacant system are first prepared during the measurement; the resonance scattering strength with wavelengths of 734nm of the two systems are measured; a working curve is drawn; then, a sample detection system is prepared; a delta I sample is obtained; and the content of the mercury in the sample is calculated according to the working curve. Compared with the conventional method, the detection method of the invention has the advantages of simple instrument, simple and convenient operation, high sensitivity, good selectivity and easily reached reaction condition; the nucleic aptamer and the Hg2+ solution used in the method have low concentrations, little reagent is used, and the cost is low.

Description

technical field [0001] The present invention relates to Hg 2+ Determination method, especially an aptamer-modified nano-gold rhenium catalysis-tellurium particle resonance scattering spectrometry for the determination of trace Hg 2+ Methods. Background technique [0002] Mercury ion is a highly toxic and widely distributed metal ion, which can damage human brain, nervous system, kidney and endothelial tissue, resulting in very serious consequences. Therefore, it is particularly important to establish a simple, fast, sensitive and selective assay method. At present, the methods for the determination of mercury ions mainly include cold atomic absorption spectrometry, atomic fluorescence spectrometry, and chemical sensor determination methods based on chromophores and fluorophores. Some of these methods have low sensitivity, and some have cumbersome steps and are not easy to operate. Contents of the invention [0003] The purpose of the present invention is to overcome th...

Claims

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Application Information

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IPC IPC(8): G01N21/75G01N21/64
Inventor 张静蒋治良温桂清刘庆业梁爱惠
Owner GUANGXI NORMAL UNIV
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