Method for extracting active ingredients of artificially cultured cordceps militaris waste liquor by flocculation

A technology of artificial cultivation and active ingredients, applied in medical preparations containing active ingredients, organic active ingredients, pharmaceutical formulations, etc., can solve the problems of increasing the difficulty of processing waste liquid and high COD value, and achieve rich recycled products and strong flocculation ability. , the effect of easy separation

Inactive Publication Date: 2013-04-24
EAST CHINA UNIV OF SCI & TECH
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Problems solved by technology

Due to the large amount of organic matter, the COD value of Cordyceps waste liquid is as high as 25,000, which increases the difficulty of waste liquid treatment

Method used

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  • Method for extracting active ingredients of artificially cultured cordceps militaris waste liquor by flocculation
  • Method for extracting active ingredients of artificially cultured cordceps militaris waste liquor by flocculation
  • Method for extracting active ingredients of artificially cultured cordceps militaris waste liquor by flocculation

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Embodiment 1

[0023] Method for Extracting Active Components from Cordyceps Waste Liquid Using Chitosan Flocculant

[0024] The molecular formula of chitosan is (C 6 h 11 NO 4 ) n , the structural formula is:

[0025]

[0026] (1) 1 gram of chitosan is dissolved in 100ml1% acetic acid solution, and the pH value of the solution is adjusted to be 5~6;

[0027] (2) At a temperature of 25°C, put the Cordyceps waste liquid in a container, add the prepared flocculant solution, the added mass fraction is 500:1, and stir further, the stirring speed is 60r / min, and the stirring time is 30 Minutes, so that the flocculant fully contacts with the macromolecules in the waste liquid to achieve a better flocculation effect;

[0028] (3) Stop stirring and stand still for 3 hours to make the flocculation and sedimentation complete; the supernatant is removed, the flocs are centrifuged, and the obtained precipitate with less water content is taken out for further processing;

[0029] (4) For the sit...

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Abstract

The invention relates to a method for extracting active ingredients of artificially cultured cordceps militaris waste liquor by flocculation, which comprises the following steps: dissolving a flocculating agent in water, wherein the mass concentration of the flocculating agent is 0.5 to 5 percent; and adjusting the pH value of the solution with acetic acid or sodium hydroxide to 3 to 9; adding the cordceps militaris waste liquor in a container at the temperature of 20 to 30 DEG C, adding the prepared flocculating agent solution into the container, and stirring at the stirring speed of 40 to 80r / min for 10 to 60min; stopping stirring, and standing the solution for 2 to 4 hours to completely settle the flocculation; removing the supernatant, centrifugalizing the flocculation, taking out a sediment containing relatively little water, and further processing the sediment; and processing the sediment to obtain a polysaccharide product. The method of the invention has the following advantages that: the processing step is simple and quick; the reclaimed products are rich and diversified; the flocculating property of the flocculating agent is strong; and the flocculation is easily separated.

Description

【Technical field】 [0001] The invention relates to the technical field of waste liquid of Cordyceps militaris, in particular to a method for flocculating and extracting effective components in waste liquid of artificially cultivated Cordyceps militaris. 【Background technique】 [0002] Cordyceps fermented liquid is rich in cordycepin, protein, amino acid, D-mannitol, Cordyceps polysaccharide, etc., among which the Cordyceps polysaccharide has strong biological activity, wide adaptability, and high development and utilization value. Due to the large amount of organic matter, the COD value of Cordyceps waste liquid is as high as 25,000, which increases the difficulty of waste liquid treatment. Therefore, finding a method that can efficiently extract the effective components in the waste liquid of Cordyceps can not only improve the economic benefits of the enterprise, but also have positive significance for environmental protection. [0003] Use flocculants to extract effective ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K31/715
Inventor 张维冰田海玉宋伦曹枫杜一平
Owner EAST CHINA UNIV OF SCI & TECH
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