Batch immersion bioreactor

Abstract

The invention discloses a batch immersion bioreactor, which comprises a biological reaction vessel, a nutrient storage vessel and a gas pump. The invention is characterized in that the upper part of the nutrient storage vessel is provided with a one-way gas valve; the nutrient storage vessel is provided with gas inlet branch pipes and liquid outlet branch pipes; the gas inlet branch pipes are provided with a first electromagnetic valve; the liquid outlet branch pipes are provided with a second electromagnetic valve; the gas inlet branch pipes are connected with a gas pump; the liquid outlet branch pipes converge to form a main pipe; the main pipe is provided with a plurality of conduits which are connected with the biological reaction vessel; the biological reaction vessel is provided with vent holes; and the horizontal position of the biological reaction vessel is higher than that of the nutrient storage vessel. The invention provides convenience for controlling the immersion time of the nutrient solution for the animal and vegetable tissues, and provides different mixture ratios of various nutrient solutions. Thus, the invention provides the nutrient combined solution respectively required by the culture of the animal and vegetable tissues, and has the advantages of energy conservation, environmental protection, high safety and reliability, and simple and convenient regulation.

Description

technical field [0001] The invention relates to a bioreactor, in particular to a batch flooding bioreactor. Background technique [0002] At present, in the field of tissue culture, the culture method mainly refers to solid or semi-solid culture in which agar is used as a support in a culture bottle. This culture method consumes a large amount of agar and has to deal with a large number of containers when filling and cleaning, so it is labor-intensive, resulting in high production costs. Although the liquid culture method has the advantages of easy renewal, no need to change containers, convenient filter sterilization, convenient cleaning, convenient application of large containers, and reduced time for seedling transfer, etc.; however, the disadvantages of liquid culture are also relatively concentrated, such as easy hypoxia, long-term immersion in Plant organs in liquid are easy to vitrify, plant organs are sensitive to stirring force, and require complex equipment. [0...

AI Technical Summary

Problems solved by technology

This culture method requires a large amount of agar and has to deal with a large number of containers when filling and cleaning, so it is labor-intensive, resulting in high production costs

Although the liquid culture method has the advantages of easy renewal, no need to change containers, convenient filter sterilization, convenient cleaning, convenient application of large containers, and reduced time for seedling transfer, etc.; however, the disadvantages of liquid culture are also relatively concentrated, such as easy hypoxia, long-term immersion in Plant organs in liquid are easy to vitrify, plant organs are sensitive to stirring force, and require complex equipment, etc.

[0003] The research on tissue culture reactors mainly includes the following aspects: improvements to traditional stirred tanks and airlift tanks, but because submerged culture is still used, the vitrification phenomenon cannot be solved; laminar flow reactors, such as shallow and transfer Drum culture reactors, although the phenomenon of vitrification is reduced, but the culture effect is not ideal; spray and atomization reactors, the culture effect of such reactors is more ideal, but this culture method grows with the growth of the tissue in the later stage of culture. , the misty culture solution is difficult to distribute evenly, which seriously affects the growth of animals and plants

[0004] The existing intermittent flooding bioreactor mainly includes a bioreactor and a nutrient storage container. The bioreactor is connected to the nutrient storage container, and the nutrient solution in the nutrient storage container is transported to the bioreactor by a water pump. The switch adjusts the contact time between the nutrient solution and the culture, thereby realizing the intermittent immersion culture of the animal and plant tissues in the nutrient solution, supplying plant nutrients when submerged, and providing sufficient oxygen for plants during intermittent periods, which better solves the problem of vitrification , and the nutrients can also be well transferred with the immersion of the plant tissue, so this culture method is a good solution to various problems in tissue culture, but the use of water pumps consumes a lot of power, and it is necessary to add a set of frequency modulation Equipment, electromechanical investment is relatively large; the pump is soaked in water during use, if there is no grounding device, once it leaks, it is very easy to cause personal injury or death; after the pump is started, it cannot quickly enter the normal working state due to the self-priming time is too long; the structure It is too complicated, and after long-term use, the deposited dirt is extremely difficult to clean up, and the repair and maintenance costs are high

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