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Protein related to abscission of plant organs and coding gene and application thereof

A plant organ, encoding gene technology, applied in the direction of plant gene improvement, application, plant peptides, etc., can solve problems such as yield loss, and achieve the effect of wide application prospects

Inactive Publication Date: 2012-11-21
CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although crops have been selected for the organ shedding trait for thousands of years, in some specific crops such as some varieties of rapeseed and cotton, there is still the problem of yield loss due to premature abscission

Method used

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  • Protein related to abscission of plant organs and coding gene and application thereof
  • Protein related to abscission of plant organs and coding gene and application thereof
  • Protein related to abscission of plant organs and coding gene and application thereof

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] Embodiment 1, preparation and confirmation and function of gene

[0036] 1. Gene preparation and confirmation

[0037] M-MLV two-step RT-PCR kit was purchased from TaKaRa, the product catalog number is D2639S. PMD-18T-easy vector was purchased from TaKaRa, catalog number D101A.

[0038] The M-MLV two-step RT-PCR kit was used for RT-PCR, and the flower organs of wild-type Arabidopsis thaliana were used as experimental materials to extract the total RNA of the tissue, which was reverse-transcribed into cDNA. The reverse transcription system was as follows:

[0039]

[0040] Incubate in a water bath at 70°C for 5 minutes, place on water for 2 minutes, centrifuge, and collect the liquid in the tube to the bottom of the tube. Add the following reagents sequentially on ice:

[0041]

[0042] Mix well, centrifuge, collect the liquid in the tube to the bottom of the tube, and perform the following reaction in the GeneAmp9700 PCR instrument: 37°C for 90min, 95°C for 5mi...

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Abstract

The invention relates to a protein related to the abscission of plant organs and a coding gene and an application thereof. The protein is the protein a) consisting of the amino acid sequence shown by sequence 1 in a sequence table, or the protein b) obtained by replacing and / or deleting and / or adding the amino acid sequence shown in the sequence 1 in the sequence table via one or a plurality of amino acid residues and coding the protein and derived from a). The gene and the action mechanism thereof can be widely used in the process of crop improvement, particularly the quality improvement of cotton and rapeseed. The gene of the invention can control the abscission progress of the plant organs without causing other changes in physiological activities, and has very wide application prospects in crop genetic breeding and quality improvement.

Description

technical field [0001] The invention relates to a protein related to plant organ shedding, its coding gene and application. Background technique [0002] The basis of harvesting crops is that the fruit does not fall off, so organ shedding can be said to be the first trait used by humans in the history of agricultural development. Although the organ shedding trait of crops has been selected for thousands of years, in some specific crops, such as some varieties of rapeseed and cotton, there is still the problem of yield loss due to premature abscission. [0003] Natural factors related to the abscission process of plants include environmental stresses such as senescence, light, and drought. The plant hormones ethylene and auxin play a regulatory role in the abscission process. According to the developmental state of the cells in the abscission zone, the abscission process of Arabidopsis floral organs can be divided into four stages: the differentiation and formation of the a...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K14/415C12N15/29C12N15/11C12N15/63C12N1/15C12N1/19C12N1/21C12N5/10A01H5/00
Inventor 王学臣魏鹏程张秀清
Owner CHINA AGRI UNIV