Method for identifying rhizoma atractylodis macrocephalae by adopting thin layer chromatography

A thin-layer chromatography method and a technique for medicinal materials, applied in the field of identification of traditional Chinese medicinal materials, can solve the problems of insufficient identification basis, inability to accurately distinguish Atractylodes atractylodes, no Atractylodes lactones, etc., achieving good popularity of instruments, equipment and materials, and avoiding the interference of impurities , the effect of simple operation

Inactive Publication Date: 2010-09-15
ZHEJIANG ACAD OF TRADITIONAL CHINESE MEDICINE
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Problems solved by technology

The thin-layer identification method with a single component as the index has shortcomings, mainly because the identification basis is not sufficient, and it cannot accurately distinguish Atractylodes macrocephala and its confusing product-Atractylodes atractylodes

Method used

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  • Method for identifying rhizoma atractylodis macrocephalae by adopting thin layer chromatography
  • Method for identifying rhizoma atractylodis macrocephalae by adopting thin layer chromatography

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Embodiment 1

[0032] 1) Weigh 2 mg of atractylodes ketone into a graduated test tube, add methanol to 2 ml, fully dissolve the atractylodes ketone, and shake well to obtain the reference substance solution of atractylodes ketone; weigh 1 mg of atractylodes Ⅰ into a graduated test tube, add methanol to 1 ml, Fully dissolve Atractylodes lactone Ⅰ, shake well, and obtain the reference substance solution of Atractylodes lactone Ⅰ; weigh 1 mg of Atractylodes lactone Ⅲ into a graduated test tube, add methanol to 1 ml, fully dissolve Atractylodes lactone Ⅲ, shake well, and obtain Atractylodes III reference substance solution.

[0033] 2) Weigh 2 g of dried Atractylodes macrocephala (produced in Pan’an, Zhejiang), put it in a conical flask, add methanol 10 times the weight of Atractylodes macrocephala, ultrasonicate for 20 minutes, let it cool, filter through ordinary filter paper, and the filtrate naturally evaporates to 2ml at room temperature. Obtain the Atractylodes Rhizome medicinal material t...

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Abstract

The invention discloses a method for identifying rhizoma atractylodis macrocephalae by adopting a thin layer chromatography. The method comprises the following steps of: taking three target compounds of atractylone, atractylenolide I and atractylenolide III as comparison products, preparing a comparison product solution by using methanol and ultrasonically extracting the rhizoma atractylodis macrocephalae by using methanol to prepare a sample solution; sucking the comparison product solution and the sample solution, respectively applying samples on the same silica gel G thin-layer plate, carrying out secondary expansion by respectively taking petroleum ether-ethyl acetate with the temperature of 60-90 DEG C and cyclohexane-ethyl acetate as expanding agents, taking out and airing, carrying out identification by visually inspecting under the conditions of natural lights and the wavelength of 365nm after color development by adopting a sulfuric acid ethanol solution, and considering the sampling medicinal material which displays same color spots as the rhizoma atractylodis macrocephalae at the same Rf (Radio frequency) position of the three comparison products. The identifying method has strong specificity as well as simple and convenient operation, can be used for accurately identifying the rhizoma atractylodis macrocephalae and definitely distinguishing the rhizoma atractylodis macrocephalae from easily-confused product-rhizoma atractylodis.

Description

technical field [0001] The invention relates to a method for identifying Atractylodes macrocephala medicinal material, in particular to a method for identifying Atractylodes macrocephala medicinal material by thin-layer chromatography. The invention belongs to the technical field of identifying traditional Chinese medicinal materials. Background technique [0002] The traditional Chinese medicine Atractylodes macrocephala is the dried rhizome of Atractylodes macrocephala (Rhizoma atractylodis macrocephala Koidz.), which is recorded in "Chinese Pharmacopoeia" 2010 edition, part one. It is a well-known traditional multi-purpose commonly used traditional Chinese medicine, and is one of the "Eight Flavors of Zhejiang". It has the effects of invigorating the spleen and replenishing qi, drying dampness and promoting diuresis, antiperspirant and preventing miscarriage. It is planted in many provinces in China, and Zhejiang Province is one of the main producing provinces. The annu...

Claims

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Application Information

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IPC IPC(8): A61K36/284G01N30/90A61P1/14A61P7/10A61P17/00A61P15/06A61K125/00
Inventor 寿旦俞忠明李洪玉章建民
Owner ZHEJIANG ACAD OF TRADITIONAL CHINESE MEDICINE
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