Preparation method of reagent plate for detecting chloramphenicol in cosmetics

A technology of reagent plate and chloramphenicol is applied in the field of preparation of immunocolloidal gold rapid detection reagent plate, which can solve the problems of high cost, unfavorable sample screening, unoptimistic situation, etc. low cost effect

Inactive Publication Date: 2010-10-13
杭州南开日新生物技术有限公司
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Problems solved by technology

[0006] For the detection of chloramphenicol in cosmetics, although the state has stepped up supervision, the situation is still not optimistic
At present, the national standard for the detection method of chloramphenicol in cosmetics is the instrument method (SN / T 2289-2009 "Determination of chloramphenicol, thiamphenicol and fluthiamphenicol in cosmetics imported and exported by liquid chromatography- Mass spectrometry / mass spectrometry"), this method has good specificity and high accuracy, but there are requirements for equipment, environment, operation skills, etc., and the cost is high, which is not conducive to large-scale sample screening

Method used

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  • Preparation method of reagent plate for detecting chloramphenicol in cosmetics
  • Preparation method of reagent plate for detecting chloramphenicol in cosmetics
  • Preparation method of reagent plate for detecting chloramphenicol in cosmetics

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Embodiment Construction

[0026] The preparation of reagent plate of the present invention comprises the preparation of chloramphenicol-carrier protein conjugate, the preparation of anti-chloramphenicol monoclonal antibody, the preparation of colloidal gold solution, the preparation of colloidal gold-labeled anti-chloramphenicol monoclonal antibody and chloramphenicol Assembly of the immunocolloidal gold rapid detection reagent plate.

[0027] 1. Coupling of chloramphenicol to carrier protein

[0028] Using carbodiimide (EDC·HCl) method, chloramphenicol was coupled with carrier protein to prepare immune antigen and coating antigen. Weigh 20 mg of bovine serum albumin (BSA), and add 1 mL of distilled water. In addition, 20 mg of EDC·HCl and 20 mg of chloramphenicol were dissolved in 1 mL of distilled water and added to the above solution. After the solution was mixed, it was placed at 4° C. and protected from light for 12 hours (inverted to mix well during this period). Afterwards, they were dialyzed...

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Abstract

The invention relates to a reagent plate for detecting immune colloidal gold of chloramphenicol, which can be used for detecting the chloramphenicol in cosmetics. The reagent plate comprises upper and lower plastic templates and a back lining, wherein a sample pad, a colloidal gold bonding pad, a nitrocellulose membrane and a water absorbing pad are closely attached to the back lining in sequence. A detection line and a quality control line are sequentially sprayed on the nitrocellulose membrane in the direction from the sample pad to the water absorbing pad so as to represent the reaction result in a macroscopic colour. The reagent plate can be used for semi-quantitative visual detection; and the entire operation process takes only 20 to 30 minutes and needs no assistance of expensive experimental facilities; therefore, the reagent plate is favourable for large-scale screening of samples and is suitable for the industry and commerce department and the inspection and quarantine organization to perform large-scale quick detection on the chloramphenicol illegally added in the cosmetics, in particular to the anti-acne and anti-acarus cosmetics.

Description

technical field [0001] The invention relates to a preparation method of a reagent plate for detecting chloramphenicol residues in cosmetics, in particular to a preparation method of an immunocolloidal gold rapid detection reagent plate for detecting chloramphenicol. Background technique [0002] In recent years, there have been frequent cases of serious adverse reactions caused by consumers using acne-removing cosmetics. For this reason, the Ministry of Health has focused on special sampling inspections for the prohibited addition of antibiotics to cosmetics. In a special sampling inspection in 2004, the Ministry of Health tested 50 kinds of cosmetics for antibacterial items. These cosmetics all claimed to have functions such as "acne removal" or "mite removal", and the test results found that 13 kinds of cosmetics were banned from adding Antibiotics were identified as unqualified products, and the unqualified rate was as high as 26%. In 2005, the Ministry of Health also la...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/531G01N33/532G01N33/577G01N33/566G01N33/558
Inventor 张少恩桑丽雅邵伟
Owner 杭州南开日新生物技术有限公司
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