The invention relates to a microfluidic
biochip for
cell high-precision arrangement and detection. The microfluidic
biochip is composed of a cover plate layer and a slide glass, wherein a charging area,
cell arrangement microchannels, an incident
optical fiber, an emergent
optical fiber or minitype photoelectric
detector, a fluid expanding area, a
cell detection area and a liquid
discharge area are arranged on the cover plate layer. Cells subjected to fluorescent
dyeing enters the microchannels from the charging area, are precisely arranged on one line through the cell arrangement microchannels, are decelerated by the fluid expanding area and sequentially pass through the cell detection area. A
laser is introduced into the cell detection area through the incident
optical fiber, and is sequentially irradiated on the cells to be detected;
fluorescence is excited, and the excited
fluorescence is guided out by the emergent optical
fiber or guided out by the minitype photoelectric
detector through
photoelectric conversion;
signal analysis is carried out to implement precise detection on the cells; and the detected cell sample solution flows out through the liquid
discharge area and is subjected to inactivation and other treatment processes. The microfluidic
biochip can implement high-precision arrangement and accurate portable detection on the cells, and has important application potential in the fields of
biomedicine,
food science and the like.