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Production method of reagent board for detecting chloramphenicol in aquatic product

A chloramphenicol and reagent plate technology, applied in the field of reagent plate preparation, can solve problems such as unfavorable large-scale sample screening, complex instrumentation, slow analysis speed, etc., and achieve the effects of low production cost, high sensitivity and mature process.

Inactive Publication Date: 2011-12-21
杭州南开日新生物技术有限公司
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Problems solved by technology

The disadvantage is that the sample pretreatment process is complex, instrumented, expensive, and the analysis speed is slow, which is not conducive to large-scale sample screening

Method used

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  • Production method of reagent board for detecting chloramphenicol in aquatic product
  • Production method of reagent board for detecting chloramphenicol in aquatic product
  • Production method of reagent board for detecting chloramphenicol in aquatic product

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Embodiment Construction

[0026] The preparation of reagent plate of the present invention comprises the preparation of chloramphenicol-carrier protein conjugate, the preparation of anti-chloramphenicol monoclonal antibody, the preparation of colloidal gold solution, the preparation of colloidal gold-labeled anti-chloramphenicol monoclonal antibody and chloramphenicol Assembly of the immunocolloidal gold rapid detection reagent plate.

[0027] 1. Coupling of chloramphenicol to carrier protein

[0028] Using carbodiimide (EDC·HCl) method, chloramphenicol was coupled with carrier protein to prepare immune antigen and coating antigen. Weigh 20 mg of bovine serum albumin (BSA), and add 1 mL of distilled water. In addition, 20 mg of EDC·HCl and 20 mg of chloramphenicol were dissolved in 1 mL of distilled water and added to the above solution. After the solution was mixed, it was placed at 4° C. and protected from light for 12 hours (inverted to mix well during this period). Afterwards, they were dialyzed...

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Abstract

The invention relates to a production method of a reagent board for detecting chloramphenicol in an aquatic product, which can be used for detecting chloramphenicol residues in the aquatic product. The reagent board consists of an upper plastic template, a lower plastic template and a back lining, wherein the back lining is sequentially tightly stuck with a sample pad, a collaurum combination pad, a nitrocellulose membrane and a water sucking pad. The nitrocellulose membrane is sprayed with a detection line and a control line sequentially in the direction from the sample pad to the water sucking pad, and a reaction result can be showed with macroscopic colors. The reagent board can be used for semi-quantitative visual detection, the whole operation process only takes 20-30min, expensive laboratory equipment is not needed for assistance, and the reagent board is conductive to large-scale sample screening, is suitable for inspection and quarantine organizations, oceanic and fishery administrations, seafood quality detection departments and the like to quickly detect chloramphenicol in illegal use in aquatic products in a large scale.

Description

technical field [0001] The invention relates to a preparation method of a reagent plate for detecting chloramphenicol residues in aquatic products, in particular to a preparation method of an immunocolloidal gold rapid detection reagent plate for detecting chloramphenicol. Background technique [0002] Chloramphenicol (CAP) is an effective broad-spectrum antibiotic, which is commonly used in the treatment of various infectious diseases in animals. It has a strong inhibitory effect on a variety of pathogenic bacteria and has been widely used in aquaculture. Also brought the serious problem of chloramphenicol residue in aquatic products. Chloramphenicol has serious side effects, can inhibit the hematopoietic function of human bone marrow, and cause human aplastic anemia, agranulocytosis, neonatal, premature infant gray syndrome and other diseases, low concentration of drug residues can also induce disease Therefore, the chloramphenicol residues in animal food constitute a hug...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/577G01N33/544G01N33/532
Inventor 张少恩桑丽雅周崇盈
Owner 杭州南开日新生物技术有限公司
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