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Enterovirus and application thereof

A technology of enteroviruses and isolates, applied in antiviral agents, viruses/bacteriophages, medical preparations containing active ingredients, etc., can solve problems such as health hazards for infants and young children

Inactive Publication Date: 2011-08-24
MICROBE EPIDEMIC DISEASE INST OF PLA MILITARY MEDICAL ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Although the disease has been reported before, it mostly appeared as sporadic cases. In 2009, it broke out in many areas of Anhui, Henan and Hebei in my country, causing serious harm to the health of infants and young children.

Method used

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  • Enterovirus and application thereof
  • Enterovirus and application thereof
  • Enterovirus and application thereof

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Embodiment 1

[0013] Embodiment 1, the acquisition of enterovirus EV71 type (Enterovirus 71) Chongqing isolate 1

[0014] 1. Isolation of enterovirus EV71 type (Enterovirus 71) Chongqing isolate 1

[0015] The inventor of the present invention collects the serum samples of patients with hand, foot and mouth disease through clinical identification, and then isolates the virus. The method of virus isolation is to inoculate the sample directly on Vero cells. When the cytopathic changes reach 95%, the virus culture supernatant is collected. After three rounds of plaque purification, a single plaque was picked, infected Vero cells for virus proliferation, and the cell suspension was collected to obtain the virus liquid. Finally, the inventor isolated an EV-71 virus from samples collected in Chongqing and named it Enterovirus EV71 type (Enterovirus 71) Chongqing isolate 1. Then the virus characteristics and pathogenicity were studied.

[0016] Enterovirus 71 type (Enterovirus 71) Chongqing isol...

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Abstract

The invention discloses an enterovirus and application thereof. The enterovirus is named Enterovirus 71 Chongqing strain 1 with the preservation and registration number of CGMCC No.3674. Experiments prove that the Enterovirus 71 Chongqing strain 1 is a weak-pathogenicity virus strain and lays a foundation for preparing attenuated vaccines in the future.

Description

technical field [0001] The invention relates to a strain of enterovirus and its application. Background technique [0002] Hand-foot-mouth disease (HFMD) is a common infectious disease caused by a variety of enteroviruses. In recent years, there have been frequent outbreaks in tropical areas of the Asia-Pacific region, mainly in infants and young children. Enteroviruses causing HFMD include enterovirus EV71 (Enterovirus 71, Enterovirus 71, referred to as EV-71), group A Coxsackie virus (CoxA), Echo virus (Echo) and the like. Among them, EV-71 is the main pathogen causing HFMD, which often leads to severe neurological syndromes. EV-71 is a single-stranded positive-sense RNA virus, which is divided into three genotypes, A, B, and C, and eight subtypes, B1-B4 and C1-C4. [0003] Although the disease had been reported before, it mostly appeared as sporadic cases. In 2009, it broke out in many areas of Anhui, Henan and Hebei in my country, causing serious harm to the health of ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N7/00A61K39/125A61P31/14C12R1/93
Inventor 常国辉吴晓燕林磊罗彦军祝庆余
Owner MICROBE EPIDEMIC DISEASE INST OF PLA MILITARY MEDICAL ACAD OF SCI