Bovine capsular serotype A Pasteurella mutocida, validation identification and application thereof

A Pasteurella, multi-kill technology, applied in the cattle-derived Capsule A type Pasteurella multocida, the application in the prevention and treatment of bovine hemorrhagic septicemia, Pasteurella pathogens and vaccine fields, can solve the economic problem of cattle industry loss, etc.

Inactive Publication Date: 2012-04-18
HARBIN VETERINARY RES INST CHINESE ACADEMY OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Because the cattle have no immunity, the disease is endemic in my country. Under severe stress conditions, the incidence rate is as high as 100%, and the fatality rate of sick cattle is as high as 40% to 60%, causing great economic losses to the cattle industry.

Method used

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  • Bovine capsular serotype A Pasteurella mutocida, validation identification and application thereof
  • Bovine capsular serotype A Pasteurella mutocida, validation identification and application thereof
  • Bovine capsular serotype A Pasteurella mutocida, validation identification and application thereof

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Embodiment 1

[0021] Example 1 Isolation and Identification of Pasteurella multocida Type A Bovine Capsule of the Present Invention

[0022] 1.1 Isolation and identification of pathogens: The pathogenic materials were obtained from lungs, livers, hearts, kidneys, respiratory tracts and intestinal tissues of affected cattle in Heilongjiang, Jilin, Ningxia, Shandong, Inner Mongolia, Tianjin and Guizhou.

[0023] After grinding the disease material, make a 1:10 suspension with sterile PBS, overnight at 4°C, take the supernatant and inoculate 3 mice intraperitoneally. The supernatant was centrifuged at 12,000×g for 10 minutes, and 3 mice were inoculated intraperitoneally. After the suspension was centrifuged, the supernatant was filtered through a 0.2um filter membrane, and 3 mice were inoculated intraperitoneally with the sterile filtrate. PBS control 2. The inoculation volume of each mouse was 0.2ml, and the incidence was observed daily. Aseptically collect the lungs, liver, heart and bloo...

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Abstract

The invention discloses bovine capsular serotype A Pasteurella mutocida and application thereof. The bovine capsular serotype A Pasteurella mutocida is separated from disease samples with haemorrhagic septicomia of cattle in six provinces (cities) with the microbial preservation number of CGMCC No.3619. The bovine capsular serotype A Pasteurella mutocida is cultured by BHI, oil adjuvant is adopted to prepare inactivated immunogen, and a mouse model proves that the bovine capsular serotype A Pasteurella mutocida has complete protecting function on serotype A Pm velogenic attck, and does not have crossing protection with serotype B Pm. Testing results prove that vaccine can be prepared by the bovine capsular serotype A Pasteurella mutocida for preventing the haemorrhagic septicomia of cattle caused by the capsular serotype A Pasteurella mutocida. The strain cannot cause diseases when inoculated to chicks, which proves that the strain is chicken isolated capsular serotype A Pasteurella mutocida.

Description

technical field [0001] The present invention relates to a strain of Pasteurella, in particular to a strain of Pasteurella multocida strain bovine-derived capsular type A, and the present invention also relates to the use of the bovine-derived capsular type A Pasteurella multocida in the prevention and treatment of bovine hemorrhagic sepsis The application of the invention belongs to the field of Pasteurella pathogens and vaccines. Background technique [0002] Pasteurella mutocida (Pasteurella mutocida, Pm) is a heterogeneous subpopulation of pathogenic bacteria, often causing diseases in domestic animals such as cattle, pigs, chickens, rabbits and wild animals, mainly sepsis and respiratory diseases. According to the antigenic specificity of the capsular polysaccharide on the surface of the bacterium, the bacterium is divided into five capsular types (A, B, D, E and F). [0003] Bovine hemorrhagic septicemia (cattle septicemia) is caused by Pasteurella multocida, an infect...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12N15/11C12Q1/68C12Q1/04A61K39/102A61P31/04C12R1/01
Inventor 于力马文戈姜志刚
Owner HARBIN VETERINARY RES INST CHINESE ACADEMY OF AGRI SCI
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