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Preparation and application of Fe-Gd double-mode magnetic resonance contrast agent with targeting function

A magnetic resonance contrast agent, dual-mode technology, applied in MRI/MRI contrast agent, emulsion delivery, drug delivery, etc., can solve the problem that normal tissue and tumor tissue cannot be distinguished, contrast material enters tumor cells, Insufficient detection and other problems, to achieve the effect of easy preparation method, uniform particle size and good biocompatibility

Inactive Publication Date: 2010-12-15
SHANGHAI NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0006] 2. Feridex is an iron oxide magnetic nanoparticle. By reducing the signal contrast, when the local magnetism is large, it will distort the image; Magnevist is a complex of gadolinium. By enhancing the signal contrast, the image is clear, but the metabolism Too fast for adequate detection;
[0007] 3. No targeting, unable to accurately make the contrast material enter the tumor cells, so that normal tissues cannot be distinguished from tumor tissues
[0011] There is no bibliographic report identical with the technology of the present invention through extensive retrieval of domestic and foreign patent documents and domestic and foreign publications

Method used

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  • Preparation and application of Fe-Gd double-mode magnetic resonance contrast agent with targeting function
  • Preparation and application of Fe-Gd double-mode magnetic resonance contrast agent with targeting function
  • Preparation and application of Fe-Gd double-mode magnetic resonance contrast agent with targeting function

Examples

Experimental program
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Embodiment 1

[0050] Prepare silica-coated water-soluble iron ferric oxide nanoparticles with a large number of amino groups on the surface.

Embodiment 2

[0053] Preparation of ferroferric oxide magnetic nanoparticles linked with diethylenepentamineacetic acid DTPA ligand.

[0054] Take 20 mg of the silica-coated water-soluble iron ferric oxide nanoparticles prepared in Step Example 1 and dissolve them in 20 ml of anhydrous dimethylformamide; add 0.45 ml of triethylamine and 5 mg of diethylene Triaminepentaacetic acid dianhydride (diethylenetriaminepentaacetic acid dianhydride); magnetic stirring, the device was sealed and evacuated, under nitrogen protection; slowly heated to 80 degrees Celsius and stirred for 1 hour, and then allowed to stand at room temperature for 10 hours; 12000 rpm centrifugal separation, with 1 % triethylamine dimethylformamide washed twice, and then washed three times with ethanol to obtain ferric oxide nanoparticles linked with diethylenepentamineacetic acid ligands.

Embodiment 3

[0056] Preparation of ferroferric oxide magnetic nanoparticles linked with gadolinium diethylenepentamine acetate Gd-DTPA.

[0057] Get 20 milligrams of ferroferric oxide magnetic nanoparticles connected to the diethylenepentamineacetic acid DTPA ligand prepared in Example 2, and dissolve it in 10 milliliters of 0.05 mol / liter tris-hydrochloric acid Tris-HCl buffer solution In, add 5 or 20 mg gadolinium nitrate hexahydrate Gd (NO 3 ) 3 ×6H 2 O, magnetic stirring, heating in an oil bath at 35 degrees Celsius for more than 12 hours; centrifugation at 12,000 rpm for the reaction solution; washing with distilled water three times to obtain ferroferric oxide magnetic nanoparticles connected with gadolinium diethylenepentamine acetate, namely iron-gadolinium T 1 , T 2 The dual-mode magnetic resonance contrast agent has a molar ratio of gadolinium element to iron element of 1.

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Abstract

The invention relates to preparation and an application of a targeted nano contrast material, in particular to preparation and an application of a Fe-Gd double-mode magnetic resonance contrast agent with a targeting function. The existing clinic magnetic resonance contrast agent has the defects of no targeting and incapacity of enabling the contrast material to access into tumor cells accurately so as not to distinguish the normal tissues from the tumor tissues. The invention adopts the method that amino groups are coupled with active groups so as to enable the water-soluble Fe3O4 to have T1 and T2 double-mode imaging and targeting functions, and can realize specificity identification for tumor cells and tissues by targeted moleculars. The invention has the advantages of simple preparation method, accurate tumor diagnosis and wide applications in fields of medicine and biology, and the contrast agent has the T1 and T2 double-mode magnetic resonance imaging and targeting functions.

Description

technical field [0001] The invention relates to the preparation and application of a targeted nano contrast material, in particular to the preparation and application of an iron-gadolinium dual-mode magnetic resonance contrast agent with a targeting function. Background technique [0002] Tumor disease is a major disease that threatens human health, and a complete cure of tumor disease is a major issue that needs to be solved urgently in the fields of contemporary medicine, chemistry and biotechnology. With the continuous deepening of tumor research, people's understanding of tumors has gradually deepened, and the therapeutic effects of many cancers have been significantly improved, but the complete treatment of cancer is still a worldwide problem. Magnetic resonance imaging is an important means of accurately diagnosing tumors. Accurate detection of common diseases such as craniocerebral; Accurate diagnosis of primary liver cancer and other tumor tissues. Multi-plane dire...

Claims

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Application Information

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IPC IPC(8): A61K49/06A61K49/18
Inventor 杨仕平庄业明杨红
Owner SHANGHAI NORMAL UNIVERSITY
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